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Literature summary for 4.1.99.2 extracted from
- Characterization of thermostable tyrosine phenol-lyase from an obligatory symbiotic thermophile, Symbiobacterium sp. SC-1 (1999), J. Biochem. Mol. Biol., 32, 480-485.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.19 | - |
L-tyrosine | pH 7.2, 60°C | Symbiobacterium sp. |  |
| 0.36 | - |
S-(o-nitrophenyl)-L-cysteine | pH 7.2, 60°C | Symbiobacterium sp. | |
| 5.5 | - |
S-methyl-L-cysteine | pH 7.2, 60°C | Symbiobacterium sp. | |
| 9.9 | - |
3,4-dihydroxyphenyl-L-alanine | pH 7.2, 60°C | Symbiobacterium sp. | |
| 12 | - |
beta-chloro-L-alanine | pH 7.2, 60°C | Symbiobacterium sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52200 | - |
4 * 52200, deduced from amino acid sequence | Symbiobacterium sp. |
| 202000 | - |
gel filtration | Symbiobacterium sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Symbiobacterium sp. | - |
SC-1 | - |
| Symbiobacterium sp. SC-1 | - |
SC-1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Symbiobacterium sp. |
Storage Stability
| Storage Stability | Organism |
|---|---|
| stable against 0.2% sodium dodecylsulfate | Symbiobacterium sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-dihydroxyphenyl-L-alanine | - |
Symbiobacterium sp. | ? | - |
? | |
| beta-chloro-L-alanine + H2O | 500% of activity compared to L-tyrosine | Symbiobacterium sp. | ? | - |
? | |
| beta-chloro-L-alanine + H2O | 500% of activity compared to L-tyrosine | Symbiobacterium sp. SC-1 | ? | - |
? | |
| D-serine + H2O | at 11% the rate of L-tyrosine | Symbiobacterium sp. | ? | - |
? | |
| D-tyrosine + H2O | at 7% the rate of L-tyrosine | Symbiobacterium sp. | phenol + pyruvate + NH3 | - |
? | |
| D-tyrosine + H2O | at 7% the rate of L-tyrosine | Symbiobacterium sp. SC-1 | phenol + pyruvate + NH3 | - |
? | |
| L-cysteine + H2O | - |
Symbiobacterium sp. | ? | - |
? | |
| L-cysteine + H2O | - |
Symbiobacterium sp. SC-1 | ? | - |
? | |
| L-serine + H2O | - |
Symbiobacterium sp. | ? | - |
? | |
| L-tyrosine + H2O | - |
Symbiobacterium sp. | phenol + pyruvate + NH3 | - |
? | |
| L-tyrosine + H2O | - |
Symbiobacterium sp. SC-1 | phenol + pyruvate + NH3 | - |
? | |
| S-(o-nitrophenyl)-L-cysteine + H2O | 250% of activity compared to L-tyrosine | Symbiobacterium sp. | ? | - |
? | |
| S-(o-nitrophenyl)-L-cysteine + H2O | 250% of activity compared to L-tyrosine | Symbiobacterium sp. SC-1 | ? | - |
? | |
| S-methyl-L-cysteine + H2O | 385% of activity compared to L-tyrosine | Symbiobacterium sp. | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 52200, deduced from amino acid sequence | Symbiobacterium sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Symbiobacterium sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Symbiobacterium sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | 1 mol per mol of subunit | Symbiobacterium sp. | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Symbiobacterium sp. | isoelectric focusing | - |
4.9 |
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