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Literature summary for 4.1.99.2 extracted from
- Partial purification and some properties of tyrosine phenol-lyase from Aeromonas phenologenes ATCC 29063 (1977), Appl. Environ. Microbiol., 33, 192-198.
General Stability
| General Stability | Organism |
|---|---|
| not stable to freezing and thawing | Aeromonas phenologenes |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
L-Tyr | - |
Aeromonas phenologenes |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 380000 | - |
gel filtration, nnon-denaturing PAGE | Aeromonas phenologenes |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aeromonas phenologenes | - |
ATCC 29063 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Aeromonas phenologenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.8 | - |
- |
Aeromonas phenologenes |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 2°C, 0.02 M phosphate buffer + mercaptoethanol, pH 7.0, 1 week, 50% loss of activity | Aeromonas phenologenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Tyr + H2O | - |
Aeromonas phenologenes | phenol + pyruvate + NH3 | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Aeromonas phenologenes |
| 50 | - |
- |
Aeromonas phenologenes |
| 60 | - |
10 min, about 45% loss of activity | Aeromonas phenologenes |
| 70 | - |
10 min, complete loss of activity | Aeromonas phenologenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Aeromonas phenologenes |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.5 | pH 7.0: about 25% of maximal activity, pH 9.5: about 60% of maximal activity | Aeromonas phenologenes |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | cofactor | Aeromonas phenologenes | |
| pyridoxal 5'-phosphate | Km: 0.032 mM | Aeromonas phenologenes | |
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