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Literature summary for 4.1.99.17 extracted from

  • Raschke, M.; Bürkle, L.; Müller, N.; Nunes-Nesi, A.; Fernie, A.R.; Arigoni, D.; Amrhein, N.; Fitzpatrick, T.B.
    Vitamin B1 biosynthesis in plants requires the essential iron sulfur cluster protein, THIC (2007), Proc. Natl. Acad. Sci. USA, 104, 19637-19642.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.99.17

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Arabidopsis thaliana 9507
-
plastid
-
 Arabidopsis thaliana 9536
-

Metals/Ions

Metals/Ions Comment Organism Structure
iron-sulfur centre
-
 Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71994
-
 x * 71994, calculated from sequence Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine Arabidopsis thaliana the enzyme is involved in the biosynthesis of thiamine diphosphate 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO
-
 ?
5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine Arabidopsis thaliana ecotype Col-0 the enzyme is involved in the biosynthesis of thiamine diphosphate 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O82392
-
-
Arabidopsis thaliana ecotype Col-0 O82392
-
-

Source Tissue

Source Tissue Comment Organism Textmining
seedling THIC transcript is not detectable two days after germination, but is readily detectable at day five Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine
-
 Arabidopsis thaliana 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO
-
 ?
5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine the enzyme is involved in the biosynthesis of thiamine diphosphate Arabidopsis thaliana 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO
-
 ?
5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine
-
 Arabidopsis thaliana ecotype Col-0 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO
-
 ?
5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine the enzyme is involved in the biosynthesis of thiamine diphosphate Arabidopsis thaliana ecotype Col-0 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO
-
 ?

Subunits

Subunits Comment Organism
? x * 71994, calculated from sequence Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
thiC
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
iron-sulfur centre
-
 Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana the THIC gene is negatively regulated by thiamin itself, regulation by conserved regions of mRNA that bind specific metabolites (riboswitches). As the thiamine riboswitch only responds to thiamin diphosphate but not thiamine, the down-regulation of THIC mRNA implies that the externally provided thiamine is converted to thiamine diphosphate inside the cell leading to the conformational change inducing mRNA instability down
Arabidopsis thaliana the transcript level in seedlings grown in 24 h of light is substantially higher than that in seedlings exposed to a single long day cycle (16-h light/8-h dark) up
Arabidopsis thaliana THIC transcript is not detectable two days after germination, but is readily detectable at day five up

General Information

General Information Comment Organism
malfunction knockdown mutant, if the thiC plants are not supplemented with thiamine, they eventually die. A concentration of 1.5 M thiamine is sufficient to allow growth of the seedlings, but these are chlorotic Arabidopsis thaliana
physiological function the enzyme is involved in the biosynthesis of thiamine diphosphate, the enzyme is essential for plant viability Arabidopsis thaliana