Cloned (Comment) | Organism |
---|---|
gene At2g22450, phylogenetic analysis, recombinant expression of His-tagged N-terminally truncated AtRIBA2 protein in Escherichia coli, recombinant expression fo GFP-tagged isozymes AtRIBA1, AtRIBA2, and AtRIBA3 in transgenic Nicotiana benthaminana chloroplasts using Agrobacterium tumefaciens pGV2260 transfection, isozymes AtRIBA2 and AtRIBA3 fail to sufficiently compensate for a lack of RIBA1 expression | Arabidopsis thaliana |
gene At5g64300, phylogenetic analysis , recombinant expression of His-tagged N-terminally truncated AtRIBA1 protein in Escherichia coli, recombinant expression of GFP-tagged isozymes AtRIBA1, AtRIBA2, and AtRIBA3 in transgenic Nicotiana benthaminana chloroplasts using Agrobacterium tumefaciens pGV2260 transfection, isozymes AtRIBA2 and AtRIBA3 fail to sufficiently compensate for a lack of RIBA1 expression | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | isozyme AtRIBA1 | Arabidopsis thaliana | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42300 | - |
x * 42300, recombinant His-tagged N-terminally truncated AtRIBA2 | Arabidopsis thaliana |
47600 | - |
x * 47600, recombinant His-tagged N-terminally truncated AtRIBA1 | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 5-phosphate | Arabidopsis thaliana | - |
formate + L-3,4-dihydroxybutan-2-one 4-phosphate | - |
? | |
D-ribulose 5-phosphate | Arabidopsis thaliana Col-0 | - |
formate + L-3,4-dihydroxybutan-2-one 4-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | P47924 | isozyme AtRIBA1, gene At5g64300 | - |
Arabidopsis thaliana | Q6NLQ7 | isozyme AtRIBA2, gene At2g22450 | - |
Arabidopsis thaliana Col-0 | P47924 | isozyme AtRIBA1, gene At5g64300 | - |
Arabidopsis thaliana Col-0 | Q6NLQ7 | isozyme AtRIBA2, gene At2g22450 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged N-terminally truncated AtRIBA1 protein from Escherichia coli by nickel affinity chromatography | Arabidopsis thaliana |
recombinant His-tagged N-terminally truncated AtRIBA2 protein from Escherichia coli by nickel affinity chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bud | - |
Arabidopsis thaliana | - |
flower | - |
Arabidopsis thaliana | - |
leaf | - |
Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
shoot | - |
Arabidopsis thaliana | - |
silique | green and yellow | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 5-phosphate | - |
Arabidopsis thaliana | formate + L-3,4-dihydroxybutan-2-one 4-phosphate | - |
? | |
D-ribulose 5-phosphate | isozyme AtRIBA1 | Arabidopsis thaliana | formate + L-3,4-dihydroxybutan-2-one 4-phosphate | - |
? | |
D-ribulose 5-phosphate | isozyme AtRIBA2 | Arabidopsis thaliana | formate + L-3,4-dihydroxybutan-2-one 4-phosphate | - |
? | |
D-ribulose 5-phosphate | - |
Arabidopsis thaliana Col-0 | formate + L-3,4-dihydroxybutan-2-one 4-phosphate | - |
? | |
D-ribulose 5-phosphate | isozyme AtRIBA1 | Arabidopsis thaliana Col-0 | formate + L-3,4-dihydroxybutan-2-one 4-phosphate | - |
? | |
D-ribulose 5-phosphate | isozyme AtRIBA2 | Arabidopsis thaliana Col-0 | formate + L-3,4-dihydroxybutan-2-one 4-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42300, recombinant His-tagged N-terminally truncated AtRIBA2 | Arabidopsis thaliana |
? | x * 47600, recombinant His-tagged N-terminally truncated AtRIBA1 | Arabidopsis thaliana |
More | both isozymes AtRIBA2 and AtRIBA3 have amino acid exchanges in conserved peptides domains that are essential for the two enzymatic functions | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
AtRIBA1 | - |
Arabidopsis thaliana |
AtRIBA2 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | angiosperms share a small RIBA gene family consisting of three members. The monofunctional, bipartite RIBA3 proteins AtRIBA2, and AtRIBA3, which have lost DHBPS activity, evolved early in tracheophyte evolution, phylogenetic analysis, overview. While AtRIBA2 is lacking GCHII activity, AtRIBA3 does not display DHBPS function | Arabidopsis thaliana |
malfunction | bleaching leaf phenotype of RIBA1 deficient plants, overview. The bleaching phenotype of the seedlings is not counterbalanced by the simultaneous AtRIBA2 and AtRIBA3 expression | Arabidopsis thaliana |
metabolism | the enzyme is involved in biosynthesis of riboflavin, overview. Riboflavin is the precursor for the synthesis of flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), which are essential cofactors for numerous enzymes (e.g., dehydrogenases, oxidases, reductases) | Arabidopsis thaliana |
additional information | in plants bifunctional RIBA enzymes comprise of N-terminal GTP cyclohydrolase II, GCHII, and C-terminal 3,4-dihydroxy-2-butanone-4-phosphate synthase, DHBPS | Arabidopsis thaliana |
physiological function | isozyme AtRIBA1 is required for pigmentation of the rosettes leaves | Arabidopsis thaliana |