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Literature summary for 4.1.99.12 extracted from

  • Hiltunen, H.M.; Illarionov, B.; Hedtke, B.; Fischer, M.; Grimm, B.
    Arabidopsis RIBA proteins: two out of three isoforms have lost their bifunctional activity in riboflavin biosynthesis (2012), Int. J. Mol. Sci., 13, 14086-14105.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene At2g22450, phylogenetic analysis, recombinant expression of His-tagged N-terminally truncated AtRIBA2 protein in Escherichia coli, recombinant expression fo GFP-tagged isozymes AtRIBA1, AtRIBA2, and AtRIBA3 in transgenic Nicotiana benthaminana chloroplasts using Agrobacterium tumefaciens pGV2260 transfection, isozymes AtRIBA2 and AtRIBA3 fail to sufficiently compensate for a lack of RIBA1 expression Arabidopsis thaliana
gene At5g64300, phylogenetic analysis , recombinant expression of His-tagged N-terminally truncated AtRIBA1 protein in Escherichia coli, recombinant expression of GFP-tagged isozymes AtRIBA1, AtRIBA2, and AtRIBA3 in transgenic Nicotiana benthaminana chloroplasts using Agrobacterium tumefaciens pGV2260 transfection, isozymes AtRIBA2 and AtRIBA3 fail to sufficiently compensate for a lack of RIBA1 expression Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast isozyme AtRIBA1 Arabidopsis thaliana 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42300
-
x * 42300, recombinant His-tagged N-terminally truncated AtRIBA2 Arabidopsis thaliana
47600
-
x * 47600, recombinant His-tagged N-terminally truncated AtRIBA1 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-ribulose 5-phosphate Arabidopsis thaliana
-
formate + L-3,4-dihydroxybutan-2-one 4-phosphate
-
?
D-ribulose 5-phosphate Arabidopsis thaliana Col-0
-
formate + L-3,4-dihydroxybutan-2-one 4-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P47924 isozyme AtRIBA1, gene At5g64300
-
Arabidopsis thaliana Q6NLQ7 isozyme AtRIBA2, gene At2g22450
-
Arabidopsis thaliana Col-0 P47924 isozyme AtRIBA1, gene At5g64300
-
Arabidopsis thaliana Col-0 Q6NLQ7 isozyme AtRIBA2, gene At2g22450
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged N-terminally truncated AtRIBA1 protein from Escherichia coli by nickel affinity chromatography Arabidopsis thaliana
recombinant His-tagged N-terminally truncated AtRIBA2 protein from Escherichia coli by nickel affinity chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
bud
-
Arabidopsis thaliana
-
flower
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
shoot
-
Arabidopsis thaliana
-
silique green and yellow Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribulose 5-phosphate
-
Arabidopsis thaliana formate + L-3,4-dihydroxybutan-2-one 4-phosphate
-
?
D-ribulose 5-phosphate isozyme AtRIBA1 Arabidopsis thaliana formate + L-3,4-dihydroxybutan-2-one 4-phosphate
-
?
D-ribulose 5-phosphate isozyme AtRIBA2 Arabidopsis thaliana formate + L-3,4-dihydroxybutan-2-one 4-phosphate
-
?
D-ribulose 5-phosphate
-
Arabidopsis thaliana Col-0 formate + L-3,4-dihydroxybutan-2-one 4-phosphate
-
?
D-ribulose 5-phosphate isozyme AtRIBA1 Arabidopsis thaliana Col-0 formate + L-3,4-dihydroxybutan-2-one 4-phosphate
-
?
D-ribulose 5-phosphate isozyme AtRIBA2 Arabidopsis thaliana Col-0 formate + L-3,4-dihydroxybutan-2-one 4-phosphate
-
?

Subunits

Subunits Comment Organism
? x * 42300, recombinant His-tagged N-terminally truncated AtRIBA2 Arabidopsis thaliana
? x * 47600, recombinant His-tagged N-terminally truncated AtRIBA1 Arabidopsis thaliana
More both isozymes AtRIBA2 and AtRIBA3 have amino acid exchanges in conserved peptides domains that are essential for the two enzymatic functions Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
AtRIBA1
-
Arabidopsis thaliana
AtRIBA2
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Arabidopsis thaliana

General Information

General Information Comment Organism
evolution angiosperms share a small RIBA gene family consisting of three members. The monofunctional, bipartite RIBA3 proteins AtRIBA2, and AtRIBA3, which have lost DHBPS activity, evolved early in tracheophyte evolution, phylogenetic analysis, overview. While AtRIBA2 is lacking GCHII activity, AtRIBA3 does not display DHBPS function Arabidopsis thaliana
malfunction bleaching leaf phenotype of RIBA1 deficient plants, overview. The bleaching phenotype of the seedlings is not counterbalanced by the simultaneous AtRIBA2 and AtRIBA3 expression Arabidopsis thaliana
metabolism the enzyme is involved in biosynthesis of riboflavin, overview. Riboflavin is the precursor for the synthesis of flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), which are essential cofactors for numerous enzymes (e.g., dehydrogenases, oxidases, reductases) Arabidopsis thaliana
additional information in plants bifunctional RIBA enzymes comprise of N-terminal GTP cyclohydrolase II, GCHII, and C-terminal 3,4-dihydroxy-2-butanone-4-phosphate synthase, DHBPS Arabidopsis thaliana
physiological function isozyme AtRIBA1 is required for pigmentation of the rosettes leaves Arabidopsis thaliana