Literature summary for 4.1.99.12 extracted from

Kumar, P.; Singh, M.; Gautam, R.; Karthikeyan, S.
Potential anti-bacterial drug target: structural characterization of 3,4-dihydroxy-2-butanone-4-phosphate synthase from Salmonella typhimurium LT2 (2010), Proteins, 78, 3292-3303.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli as a His-tagged fusion protein	Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

Crystallization (Comment)	Organism
the crystal structures of Salmonella DHBPS in complex with sulfate, D-ribulose 5-phosphate and sulfate-zinc ion is determined at a resolution of 2.80, 2.52, and 1.86 A, respectively. Analysis of these crystal structures reveals that the acidic loop (residues 34-39) responsible for the acid-base catalysis is disordered in the absence of substrate or metal ion at the active site. Upon binding either substrate or sulfate and metal ions, the acidic loop becomes stabilized, adopts a closed conformation and interacts with the substrate	Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Comment)

Organism

the crystal structures of Salmonella DHBPS in complex with sulfate, D-ribulose 5-phosphate and sulfate-zinc ion is determined at a resolution of 2.80, 2.52, and 1.86 A, respectively. Analysis of these crystal structures reveals that the acidic loop (residues 34-39) responsible for the acid-base catalysis is disordered in the absence of substrate or metal ion at the active site. Upon binding either substrate or sulfate and metal ions, the acidic loop becomes stabilized, adopts a closed conformation and interacts with the substrate

Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.116	-	D-ribulose 5-phosphate	37°C, pH 7.5, Vmax: 199 nmol/min/mg	Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions	Comment	Organism
Hg2+	moderate activity	Salmonella enterica subsp. enterica serovar Typhimurium
Mg2+	highest activity	Salmonella enterica subsp. enterica serovar Typhimurium
Ni2+	moderate activity	Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
23310	-	calculated from cDNA	Salmonella enterica subsp. enterica serovar Typhimurium
23640	-	MALDI	Salmonella enterica subsp. enterica serovar Typhimurium
50000	-	gel filtration, dimer in solution	Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism	UniProt	Comment	Textmining
Salmonella enterica subsp. enterica serovar Typhimurium	P66032	-	-

Purification (Commentary)

Purification (Comment)	Organism
using Ni-NTA chromatography	Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-ribulose 5-phosphate	-	Salmonella enterica subsp. enterica serovar Typhimurium	formate + L-3,4-dihydroxy-2-butanone-4-phosphate	-	?

Subunits

Subunits	Comment	Organism
homodimer	gel filtration, 2 * 23310	Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

Synonyms	Comment	Organism
3,4-dihydroxy-2-butanone-4-phosphate synthase	-	Salmonella enterica subsp. enterica serovar Typhimurium
DHBPS	-	Salmonella enterica subsp. enterica serovar Typhimurium
ribB	-	Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Salmonella enterica subsp. enterica serovar Typhimurium