Literature summary for 4.1.99.12 extracted from

Liao, D.I.; Calabrese, J.C.; Wawrzak, Z.; Viitanen, P.V.; Jordan, D.B.
Crystal structure of 3,4-dihydroxy-2-butanone 4-phosphate synthase of riboflavin biosynthesis (2001), Structure, 9, 11-18.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, hanging drop vapour diffusion method at room temperature, 4-5 days, 0.0022 ml of protein solution containing 24 mg/ml protein in 50 mM Tris-HCl pH 7.5, is mixed with 0.0007 ml of precipitating well solution containing 3 M CsCl, 3 M Cs-formate, 20 mM Bis-Tris-propane-NaOH, pH 6.9, or 6 M sodium formate, 25 mM HEPES-NaOH, pH 7.0, labeling with 1.5 mM Au(CN)2, X-ray diffraction structure determination and analysis at 1.4-2.4 A resolution, multiwavelength anomalous diffraction	Escherichia coli

Crystallization (Comment)

Organism

purified recombinant enzyme, hanging drop vapour diffusion method at room temperature, 4-5 days, 0.0022 ml of protein solution containing 24 mg/ml protein in 50 mM Tris-HCl pH 7.5, is mixed with 0.0007 ml of precipitating well solution containing 3 M CsCl, 3 M Cs-formate, 20 mM Bis-Tris-propane-NaOH, pH 6.9, or 6 M sodium formate, 25 mM HEPES-NaOH, pH 7.0, labeling with 1.5 mM Au(CN)2, X-ray diffraction structure determination and analysis at 1.4-2.4 A resolution, multiwavelength anomalous diffraction

Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required for activity	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-ribulose 5-phosphate	Escherichia coli	the enzyme is involved in the pathway of riboflavin biosynthesis, overview	formate + L-3,4-dihydroxy-2-butanone-4-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0A7J0	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate	catalytic reaction mechanism, substrate recognition and active site structure, active site consists of three glutamates, two aspartates, two histidines, and a cysteine which may provide the means for general acid and base catalysis and for coordinating the Mg2+ cofactor within the active site	Escherichia coli	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-ribulose 5-phosphate	-	Escherichia coli	formate + L-3,4-dihydroxy-2-butanone-4-phosphate	-	?
D-ribulose 5-phosphate	the enzyme is involved in the pathway of riboflavin biosynthesis, overview	Escherichia coli	formate + L-3,4-dihydroxy-2-butanone-4-phosphate	-	?

Subunits

Subunits	Comment	Organism
More	structure determination and analysis of functions of single residues, overview, dimer interface structure, localization of the active site, sequence and structure comparison	Escherichia coli

Synonyms

Synonyms	Comment	Organism
3,4-dihydroxy-2-butanone 4-phosphate synthase	-	Escherichia coli