Cloned (Comment) | Organism |
---|---|
gene ribB, overexpression in an rib-defective mutant strain | Escherichia coli K-12 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | complete inhibition | Escherichia coli K-12 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli K-12 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23251 | - |
x * 23251, recombinant enzyme, mass spectrometry | Escherichia coli K-12 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 5-phosphate | Escherichia coli K-12 | the enzyme is important in the riboflavin biosynthesis, the riboflavin precursor, 6,7-dimethyl-8-ribityllumazine, is formed by condensation of 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione (1) with 3,4-dihydroxy-2-butanone 4-phosphate, overview | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli K-12 | P0A7J0 | gene ribB | - |
Purification (Comment) | Organism |
---|---|
native enzyme by anion and cation exchange chromatography, hydroxyapatite chromatography, and gel filtration, to homogeneity, recombinant enzyme from the overexpressing strain by anion exchange chromatography and gel filtration | Escherichia coli K-12 |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate | catalytic mechanism | Escherichia coli K-12 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.283 | - |
purified recombinant enzyme, assay method development, overview | Escherichia coli K-12 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 5-phosphate | substrate preparation, overview | Escherichia coli K-12 | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
D-ribulose 5-phosphate | the enzyme is important in the riboflavin biosynthesis, the riboflavin precursor, 6,7-dimethyl-8-ribityllumazine, is formed by condensation of 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione (1) with 3,4-dihydroxy-2-butanone 4-phosphate, overview | Escherichia coli K-12 | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23251, recombinant enzyme, mass spectrometry | Escherichia coli K-12 |
Synonyms | Comment | Organism |
---|---|---|
DHBP synthase | - |
Escherichia coli K-12 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli K-12 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli K-12 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor required | Escherichia coli K-12 |