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Literature summary for 4.1.99.12 extracted from

  • Steinbacher, S.; Schiffmann, S.; Bacher, A.; Fischer, M.
    Metal sites in 3,4-dihydroxy-2-butanone 4-phosphate synthase from Methanococcus jannaschii in complex with the substrate ribulose 5-phosphate (2004), Acta Crystallogr. Sect. D, 60, 1338-1340.
    View publication on PubMed
Search for more literature for 4.1.99.12

Crystallization (Commentary)

Crystallization (Comment) Organism
purified enzyme mutant H147S in complex with substrate ribulose 5-phosphate, monoclinic crystal form, X-ray diffraction structure determination and analysis at 1.55-1.7 A resolution Methanocaldococcus jannaschii

Protein Variants

Protein Variants Comment Organism
H147S site-directed mutagenesis, the mutant shows about 10% of wild-type enzyme activity, crystal structure determination with bound substrate Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Ca2+ binding requires the binding of the substrate, binding involves Glu204, Glu97, and Asn207 Methanocaldococcus jannaschii
additional information substrate binding structure involving a dimetal center, overview Methanocaldococcus jannaschii
Zn2+ bound at His164, binding involves His206, Glu132, and Glu128 Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-ribulose 5-phosphate Methanocaldococcus jannaschii DHBPS supplies the building blocks for the assembly of the xylene ring of the vitamin B2, riboflavin, all eight C atoms of the xylene moiety are derived from the product of the enzyme formate + L-3,4-dihydroxy-2-butanone-4-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii
-
-
-

Reaction

Reaction Comment Organism Reaction ID
D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate active site architecture Methanocaldococcus jannaschii
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribulose 5-phosphate DHBPS supplies the building blocks for the assembly of the xylene ring of the vitamin B2, riboflavin, all eight C atoms of the xylene moiety are derived from the product of the enzyme Methanocaldococcus jannaschii formate + L-3,4-dihydroxy-2-butanone-4-phosphate
-
 ?
D-ribulose 5-phosphate substrate binding structure involving a dimetal center, overview Methanocaldococcus jannaschii formate + L-3,4-dihydroxy-2-butanone-4-phosphate the enzyme converts ribulose 5-phosphate into 3,4-dihydroxy-2-butanone 4-phosphate, while its C4 atom is released as formate ?

Subunits

Subunits Comment Organism
dimer enzyme mutant H147S, crystal structure Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
3,4-dihydroxy-2-butanone 4-phosphate synthase
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 Methanocaldococcus jannaschii
DHBPS
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 Methanocaldococcus jannaschii