Literature summary for 4.1.99.12 extracted from

Liao, D.I.; Viitanen, P.V.; Jordan, D.B.
Cloning, expression, purification and crystallization of dihydroxybutanone phosphate synthase from Magnaporthe grisea (2000), Acta Crystallogr. Sect. D, 56, 1495-1497.

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Cloned(Commentary)

Cloned (Comment)	Organism
cloning by functional complementation of an Escherichia coli DS knockout mutant, expression in Escherichia coli strain BL21(DE3)	Pyricularia grisea

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinnat enzyme divalent cation free, soaked in Zn2+ or soaked in Mg2+, hanging drop vapor diffusion method, room temperature, 0.001 ml of 7 mg/ml protein in 50 mM Tris-HCl, pH 7.5, is mixed with 0.001 ml well solution containing 24-30% PEG monomethyl ether, 0.2 M Li2SO4, and 0.1 M MES-NaOH, pH 6.0-6.5, 1 week-3 months, rectangular plates, X-ray diffraction structure determination and analysis at 1.5 A, 1.0 A, and 1.8 A resolution, respectively	Pyricularia grisea

Crystallization (Comment)

Organism

purified recombinnat enzyme divalent cation free, soaked in Zn2+ or soaked in Mg2+, hanging drop vapor diffusion method, room temperature, 0.001 ml of 7 mg/ml protein in 50 mM Tris-HCl, pH 7.5, is mixed with 0.001 ml well solution containing 24-30% PEG monomethyl ether, 0.2 M Li2SO4, and 0.1 M MES-NaOH, pH 6.0-6.5, 1 week-3 months, rectangular plates, X-ray diffraction structure determination and analysis at 1.5 A, 1.0 A, and 1.8 A resolution, respectively

Pyricularia grisea

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-ribulose 5-phosphate	Pyricularia grisea	-	formate + L-3,4-dihydroxy-2-butanone-4-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Pyricularia grisea	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange and hydroxylapatite chromatography, followed by ultrafiltration	Pyricularia grisea

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-ribulose 5-phosphate	-	Pyricularia grisea	formate + L-3,4-dihydroxy-2-butanone-4-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
dihydroxybutanone phosphate synthase	-	Pyricularia grisea