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Literature summary for 4.1.3.39 extracted from

  • Manjasetty, B.A.; Croteau, N.; Powlowski, J.; Vrielink, A.
    Crystallization and preliminary x-ray analysis of dmpFG-encoded 4-hydroxy-2-ketovalerate aldolase-aldehyde dehydrogenase (acylating) from Pseudomonas sp. strain CF600 (2001), Acta Crystallogr. Sect. D, 57, 582-585.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
purified enzyme complex of 4-hydroxy-2-ketovalerate aldolase and aldehyde dehydrogenase (acylating), protein solution contains 9 mg/ml protein in 50 mM Tris-HCl, pH 7.4, 1 mM DTT, and 2 mM NAD+, mixing of equal volumes of 0.001 ml of protein and reservoir solutions, the latter contains 15% PEG 8000, 100 mM ammonium sulfate, and 100 mM PIPES, pH 7.5, 3 days, soaking of crystals in 5 mM samarium acetate and 0.25 mM PCMBS, X-ray diffraction structure determination and analysis at 2.1 A resolution Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-hydroxy-2-oxovalerate Pseudomonas sp. last but one step in meta-cleavage pathway for catechol degradation pyruvate + acetaldehyde
-
?
4-hydroxy-2-oxovalerate Pseudomonas sp. CF 600 last but one step in meta-cleavage pathway for catechol degradation pyruvate + acetaldehyde
-
?
additional information Pseudomonas sp. the enzyme acts in an enzyme complex with the aldehyde dehydrogenase, EC 1.2.1.10, performs the final step in meta-cleavage pathway for catechol degradation ?
-
?
additional information Pseudomonas sp. CF 600 the enzyme acts in an enzyme complex with the aldehyde dehydrogenase, EC 1.2.1.10, performs the final step in meta-cleavage pathway for catechol degradation ?
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
dmpFG-encoded 4-hydroxy-2-ketovalerate aldolase/aldehyde dehydrogenase (acylating), EC 1.2.1.10
-
Pseudomonas sp. CF 600
-
dmpFG-encoded 4-hydroxy-2-ketovalerate aldolase/aldehyde dehydrogenase (acylating), EC 1.2.1.10
-

Purification (Commentary)

Purification (Comment) Organism
co-purification with aldehyde dehydrogenase, EC 1.2.1.10, to homogeneity in a 3-step chromatographic procedure involving NAD+ affinity chromatography Pseudomonas sp.

Reaction

Reaction Comment Organism Reaction ID
(S)-4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate reaction mechanism Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxy-2-oxovalerate
-
Pseudomonas sp. pyruvate + acetaldehyde
-
?
4-hydroxy-2-oxovalerate last but one step in meta-cleavage pathway for catechol degradation Pseudomonas sp. pyruvate + acetaldehyde
-
?
4-hydroxy-2-oxovalerate
-
Pseudomonas sp. CF 600 pyruvate + acetaldehyde
-
?
4-hydroxy-2-oxovalerate last but one step in meta-cleavage pathway for catechol degradation Pseudomonas sp. CF 600 pyruvate + acetaldehyde
-
?
additional information the enzyme acts in an enzyme complex with the aldehyde dehydrogenase, EC 1.2.1.10, performs the final step in meta-cleavage pathway for catechol degradation Pseudomonas sp. ?
-
?
additional information the enzyme acts in an enzyme complex with the aldehyde dehydrogenase, EC 1.2.1.10, which acts on acetaldehyde to form acetyl-CoA, product channeling mechanism Pseudomonas sp. ?
-
?
additional information the enzyme acts in an enzyme complex with the aldehyde dehydrogenase, EC 1.2.1.10, performs the final step in meta-cleavage pathway for catechol degradation Pseudomonas sp. CF 600 ?
-
?
additional information the enzyme acts in an enzyme complex with the aldehyde dehydrogenase, EC 1.2.1.10, which acts on acetaldehyde to form acetyl-CoA, product channeling mechanism Pseudomonas sp. CF 600 ?
-
?

Subunits

Subunits Comment Organism
More the enzyme forms a heterodimer of 71 kDa with the aldehyde dehydrogenase, EC 1.2.1.10 Pseudomonas sp.

Synonyms

Synonyms Comment Organism
DmpFG
-
Pseudomonas sp.