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Literature summary for 4.1.3.30 extracted from

  • Limenitakis, J.; Oppenheim, R.D.; Creek, D.J.; Foth, B.J.; Barrett, M.P.; Soldati-Favre, D.
    The 2-methylcitrate cycle is implicated in the detoxification of propionate in Toxoplasma gondii (2013), Mol. Microbiol., 87, 894-908.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.3.30

Cloned(Commentary)

Cloned (Comment) Organism
gene prpB, phylogenetic analysis, C-terminally Ty-tagged version of TgPrpB is located in the cytosolic fraction whereas an N-terminally myc-tagged TgPrpB is partitioned in both the cytosol and organellar fractions, mycPrpB is produced as a precursor of about 40 kDa that corresponds to the predicted size of full-length PrpB and this form is detectable with anti-myc Toxoplasma gondii

Protein Variants

Protein Variants Comment Organism
additional information TgPrpB gene disrupture by double homologous gene replacement in RH tachyzoites. The enzyme-deficient prpb-ko mutant is complemented by expression of wild-type myc-tagged TgPrpB Toxoplasma gondii

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol exclusively, subcellular localization analysis Toxoplasma gondii 5829
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 x * 40000, about, recombinant N-terminally myc-tagged pro-PrpB, SDS-PAGE Toxoplasma gondii

Organism

Organism UniProt Comment Textmining
Toxoplasma gondii Q2L8W6 gene prpB
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Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification recombinant N-terminally myc-tagged PrpB is produced as a precursor of about 40 kDa Toxoplasma gondii

Source Tissue

Source Tissue Comment Organism Textmining
tachyzoite
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 Toxoplasma gondii
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Subunits

Subunits Comment Organism
? x * 40000, about, recombinant N-terminally myc-tagged pro-PrpB, SDS-PAGE Toxoplasma gondii

Synonyms

Synonyms Comment Organism
2-Methylisocitrate lyase
-
 Toxoplasma gondii
TgPrpB
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 Toxoplasma gondii

General Information

General Information Comment Organism
evolution the 2-methylcitrate cycle enzymes have been acquired via horizontal gene transfer, PrpB phylogenetic analysis, overview Toxoplasma gondii
malfunction an enzyme-disruption mutant prpb-ko shows accumulation of 2-methylcitrate cycle intermediate metabolites, enzyme substrates accumulate in parasites deficient in the enzyme and its absence confers increased sensitivity to propionic acid Toxoplasma gondii
physiological function the enzyme is involved in the 2-methylcitrate cycle required to metabolize and detoxify propionate by oxidation to pyruvate, but the rate-limiting enzyme 2-methylisocitrate lyase is dispensable for parasite survival Toxoplasma gondii