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Literature summary for 4.1.3.30 extracted from

  • Brock, M.; Darley, D.; Textor, S.; Buckel, W.
    2-Methylisocitrate lyases from the bacterium Escherichia coli and the filamentous fungus Aspergillus nidulans: characterization and comparison of both enzymes (2001), Eur. J. Biochem., 268, 3577-3586.
    View publication on PubMed
Search for more literature for 4.1.3.30

General Stability

General Stability Organism
enzyme becomes inactivated when incubated with oxygen, partial reactivation with dithiothreitol possible Aspergillus nidulans

Inhibitors

Inhibitors Comment Organism Structure
3-Bromopyruvate complete inactivation at equimolar concentrations Aspergillus nidulans
Ca2+ 40% inactivation at 2 mM; inhibition at 2 mM Aspergillus nidulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
19
-
 (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate pH 7 Aspergillus nidulans
31
-
 (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate pH 7 Aspergillus nidulans

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent, 2 mM, Km: 58 mM at pH 7 Aspergillus nidulans
Mg2+ dependentm Km: 35 mM at pH 7 Aspergillus nidulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 4 * 32000, SDS-PAGE Aspergillus nidulans
66000
-
 4 * 66000, SDS-PAGE Aspergillus nidulans
130000
-
 gel filtration Aspergillus nidulans
244000
-
 gel filtration Aspergillus nidulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Aspergillus nidulans enzyme in methylcitrate cycle ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans
-
-
-

Oxidation Stability

Oxidation Stability Organism
enzyme becomes inactivated when incubated with oxygen, partial reactivation with dithiothreitol possible Aspergillus nidulans

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus nidulans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21.4
-
-
 Aspergillus nidulans
91
-
-
 Aspergillus nidulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate syn: methylisocitrate, enzyme acts only on threo-diastereomer, but not on the erythro-diastereomer Aspergillus nidulans Pyruvate + succinate
-
 ?
additional information no substrate: isocitrate Aspergillus nidulans ?
-
 ?
additional information enzyme in methylcitrate cycle Aspergillus nidulans ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 66000, SDS-PAGE Aspergillus nidulans
tetramer 4 * 32000, SDS-PAGE Aspergillus nidulans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
-
 stable for several days Aspergillus nidulans
40
-
 stable for 60 min Aspergillus nidulans
50
-
 half-life: 15 min Aspergillus nidulans
50
-
 complete inactivation after 15 min Aspergillus nidulans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12.3
-
 (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate pH 7 Aspergillus nidulans
100
-
 (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate pH 7 Aspergillus nidulans