Application | Comment | Organism |
---|---|---|
biotechnology | development of a microbial system for the environmentally-compatible synthesis of anthranilate generated by metabolic engineering of trpD gene from strain W3110 trpD9923 | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
W3110 trpD9923 | - |
Escherichia coli W3110 trpD9923 | - |
W3110 trpD9923 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chorismate + L-glutamine | - |
Escherichia coli | anthranilate + pyruvate + L-glutamate | - |
? | |
chorismate + L-glutamine | - |
Escherichia coli W3110 trpD9923 | anthranilate + pyruvate + L-glutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
anthranilate synthase | - |
Escherichia coli |
TrpED | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | mutation in strain trpD9923 (mutant in the tryptophan operon) results in the synthesis of a truncated anthranilate synthase component II protein, retaining the full glutamine amidotransferase domain and only seven of the 333 amino acid residues of the anthranilate phosphoribosyl transferase domain. Mutation in the trpD gene causes the loss of anthranilate phosphoribosyl transferase activity, but maintains anthranilate synthase activity, thus causing anthranilate accumulation | Escherichia coli |