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Literature summary for 4.1.3.27 extracted from

  • Kanno, T.; Komatsu, A.; Kasai, K.; Dubouzet, J.G.; Sakurai, M.; Ikejiri-Kanno, Y.; Wakasa, K.; Tozawa, Y.
    Structure-based in vitro engineering of the anthranilate synthase, a metabolic key enzyme in the plant tryptophan pathway (2005), Plant Physiol., 138, 2260-2268.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.3.27

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Oryza sativa

Protein Variants

Protein Variants Comment Organism
A369L Trp-insensitive mutant of anthranilate synthase alpha-subunit OASA2 Oryza sativa
A380S mutation of anthranilate synthase alpha-subunit OASA2 enhances catalytic activity Oryza sativa
A532Y mutation of anthranilate synthase alpha-subunit OASA2 abolishes activity Oryza sativa
G518A mutation of anthranilate synthase alpha-subunit OASA2 abolishes activity Oryza sativa
G521A Trp-insensitive mutant of anthranilate synthase alpha-subunit OASA2 Oryza sativa
G522A increased sensitivity to Trp feedback inhibition of anthranilate synthase alpha-subunit OASA2 mutant Oryza sativa
G522Y increased sensitivity to Trp feedback inhibition of anthranilate synthase alpha-subunit OASA2 mutant Oryza sativa
L520F mutation of anthranilate synthase alpha-subunit OASA2 enhances catalytic activity Oryza sativa
L530D mutation of anthranilate synthase alpha-subunit OASA2 enhances catalytic activity Oryza sativa
N351A increased sensitivity to Trp feedback inhibition of anthranilate synthase alpha-subunit OASA2 mutant Oryza sativa
N351D mutation of anthranilate synthase alpha-subunit OASA2 enhances catalytic activity Oryza sativa
N363A mutation of anthranilate synthase alpha-subunit OASA2 abolishes activity Oryza sativa
N363D mutation of anthranilate synthase alpha-subunit OASA2 abolishes activity Oryza sativa
P364A mutation of anthranilate synthase alpha-subunit OASA2 abolishes activity Oryza sativa
P364L mutation of anthranilate synthase alpha-subunit OASA2 abolishes activity Oryza sativa
P366A mutation of anthranilate synthase alpha-subunit OASA2 abolishes activity Oryza sativa
S126A Trp-insensitive mutant of anthranilate synthase alpha-subunit OASA2 Oryza sativa
S356A Trp-insensitive mutant of anthranilate synthase alpha-subunit OASA2 Oryza sativa
Y349A mutation of anthranilate synthase alpha-subunit OASA2 enhances catalytic activity Oryza sativa
Y349F mutation of anthranilate synthase alpha-subunit OASA2 enhances catalytic activity Oryza sativa
Y367A Trp-insensitive mutant of anthranilate synthase alpha-subunit OASA2 Oryza sativa
Y367A/L530D accumulation of free Trp in cells expressing the mutant OASA2 is 2.3fold of that of cells expressing wild-type D126F /L530D Oryza sativa
Y516A nonfunctional protein, anthranilate synthase alpha-subunit OASA2 Oryza sativa

Inhibitors

Inhibitors Comment Organism Structure
L-Trp feedback-inhibition, IC50: 0.0063 mM for wild-type enzyme, 0.0933 mM for mutant S126F, 0.0316 mM for mutant Y367A, 0.0749 mM for mutant A369L, 0.101 mM for mutant Y367A/L530D and 0.0583 mM for mutant A369L/L530D Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.039
-
 chorismate pH 8.3, 32°C, A369L/L530D mutant anthranilate synthase alpha-subunit OASA2 Oryza sativa
0.043
-
 chorismate pH 8.3, 32°C, A369L mutant anthranilate synthase alpha-subunit OASA2 Oryza sativa
0.043
-
 chorismate pH 8.3, 32°C, wild-type enzyme, anthranilate synthase alpha-subunit OASA2 Oryza sativa
0.049
-
 chorismate pH 8.3, 32°C, S126F mutant anthranilate synthase alpha-subunit OASA2 Oryza sativa
0.058
-
 chorismate pH 8.3, 32°C, Y367A/L530D mutant anthranilate synthase alpha-subunit OASA2 Oryza sativa
0.062
-
 chorismate pH 8.3, 32°C, S126F/L530D mutant anthranilate synthase alpha-subunit OASA2 Oryza sativa
0.076
-
 chorismate pH 8.3, 32°C, Y367A mutant anthranilate synthase alpha-subunit OASA2 Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chorismate + NH4+
-
 Oryza sativa anthranilate + pyruvate + H2O
-
 ?

Synonyms

Synonyms Comment Organism
OASA2 anthranilate synthase alpha-subunit Oryza sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.092
-
 chorismate pH 8.3, 32°C, Y367A mutant anthranilate synthase alpha-subunit OASA2 Oryza sativa
0.108
-
 chorismate pH 8.3, 32°C, S126F mutant anthranilate synthase alpha-subunit OASA2 Oryza sativa
0.22
-
 chorismate pH 8.3, 32°C, A369L mutant anthranilate synthase alpha-subunit OASA2 Oryza sativa
0.225
-
 chorismate pH 8.3, 32°C, wild-type enzyme, anthranilate synthase alpha-subunit OASA2 Oryza sativa
0.405
-
 chorismate pH 8.3, 32°C, A369L/L530D mutant anthranilate synthase alpha-subunit OASA2 Oryza sativa
0.49
-
 chorismate pH 8.3, 32°C, Y367A/L530D mutant anthranilate synthase alpha-subunit OASA2 Oryza sativa
0.573
-
 chorismate pH 8.3, 32°C, S126F/L530D mutant anthranilate synthase alpha-subunit OASA2 Oryza sativa
6.08
-
 chorismate pH 8.3, 32°C, S126F/L530D mutant anthranilate synthase alpha-subunit OASA2 Oryza sativa

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0063
-
 feedback-inhibition, IC50: 0.0063 mM for wild-type enzyme, 0.0933 mM for mutant S126F, 0.0316 mM for mutant Y367A, 0.0749 mM for mutant A369L, 0.101 mM for mutant Y367A/L530D and 0.0583 mM for mutant A369L/L530D Oryza sativa L-Trp