Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Archaeoglobus fulgidus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-Trp | competitive with respect to chorismate | Archaeoglobus fulgidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0123 | - |
chorismate | pH 8.0, 60°C, without glycerol and KCl | Archaeoglobus fulgidus | |
0.084 | - |
chorismate | pH 8.0, 60°C, with 25% glycerol and 2 M KCl | Archaeoglobus fulgidus | |
5.7 | - |
NH4+ | pH 8.0, 60°C, without glycerol and KCl | Archaeoglobus fulgidus | |
6.2 | - |
L-Gln | pH 8.0, 60°C, without glycerol and KCl | Archaeoglobus fulgidus | |
7 | - |
L-Gln | pH 8.0, 60°C, with 25% glycerol and 2 M KCl | Archaeoglobus fulgidus | |
7.3 | - |
NH4+ | pH 8.0, 60°C, with 25% glycerol and 2 M KCl | Archaeoglobus fulgidus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
alpha2beta2, 2 * 18000 + 2 * 50000, SDS-PAGE | Archaeoglobus fulgidus |
50000 | - |
alpha2beta2, 2 * 18000 + 2 * 50000, SDS-PAGE | Archaeoglobus fulgidus |
150000 | - |
gel filtration | Archaeoglobus fulgidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Archaeoglobus fulgidus |
Storage Stability | Organism |
---|---|
-20°C, in a 1:1 mixture of glycerol, 50 mM tricine, pH 7.5,1 mM DTT, 0.1 mM EDTA, additional protein bands appear upon storage, after an initial 10 15% drop in activity, little or no loss of enzyme activity is observed over a period of several weeks | Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chorismate + L-Gln | - |
Archaeoglobus fulgidus | anthranilate + pyruvate + L-glutamate | - |
? | |
chorismate + NH4+ | - |
Archaeoglobus fulgidus | anthranilate + pyruvate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha2beta2, 2 * 18000 + 2 * 50000, SDS-PAGE | Archaeoglobus fulgidus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
half-life in presence of 25% glycerol without KCl: 4.6 min, in 25% glycerol with 2 M KCl activity is constant for over 4 h after initial drop in activity to 83% | Archaeoglobus fulgidus |
80 | - |
half-life in presence of 25% glycerol without KCl: 1.2 min, half-life in presence of 25% glycerol and 2 M KCl: 200 min | Archaeoglobus fulgidus |
85 | - |
half-life in presence of 25% glycerol without KCl: 0.9 min, half-life in presence of 25% glycerol and 2 M KCl: 50 min | Archaeoglobus fulgidus |
90 | - |
half-life in presence of 25% glycerol and 2 M KCl: 12 min | Archaeoglobus fulgidus |
95 | - |
half-life in presence of 25% glycerol and 2 M KCl: 4.1 min | Archaeoglobus fulgidus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
chorismate | pH 8.0, 60°C, with or without 2 M KCl and 25% glycerol | Archaeoglobus fulgidus | |
0.25 | - |
L-Gln | pH 8.0, 60°C, with or without 2 M KCl and 25% glycerol | Archaeoglobus fulgidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
NH4+-dependent reaction, with 25% glycerol and 2 M KCl | Archaeoglobus fulgidus |
8.1 | - |
L-Gln-dependent reaction, with 25% glycerol and 2 M KCl | Archaeoglobus fulgidus |
8.7 | - |
NH4+-dependent reaction, without glycerol and KCl | Archaeoglobus fulgidus |
8.8 | - |
L-Gln+-dependent reaction, without glycerol and KCl | Archaeoglobus fulgidus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00028 | - |
L-Trp | pH 8.0, 60°C, without glycerol and KCl | Archaeoglobus fulgidus | |
0.00031 | - |
L-Trp | pH 8.0, 60°C, with 25% glycerol and 2 M KCl | Archaeoglobus fulgidus |