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Literature summary for 4.1.3.27 extracted from
- Anthranilate synthase from Pseudomonas putida. Purification and properties of a two-component enzyme (1973), J. Biol. Chem., 248, 151-161.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 6-diazo-5-oxo-L-norleucine | - |
Pseudomonas putida |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.007 | - |
chorismate | enzyme component ASI, with Gln as cosubstrate | Pseudomonas putida | |
| 0.09 | - |
chorismate | two-component enzyme, ASI,ASII, with ammonium as cosubstrate | Pseudomonas putida | |
| 0.6 | - |
Gln | - |
Pseudomonas putida | |
| 26 | - |
NH4+ | two-component enzyme, ASI,ASII | Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 18000 | - |
1 * 18000 + 1 * 63400, sucrose density gradient centrifugation | Pseudomonas putida |
| 63400 | - |
1 * 18000 + 1 * 63400, sucrose density gradient centrifugation | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chorismate + L-Gln | - |
Pseudomonas putida | anthranilate + pyruvate + L-glutamate | - |
? | |
| chorismate + NH4+ | reaction is catalyzed by component I alone and in combination with component II | Pseudomonas putida | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 18000 + 1 * 63400, sucrose density gradient centrifugation | Pseudomonas putida |
| More | glutamine, 10 mM, enhances binding of AS I and II, as does Mg2+. ASI and ASII play a joint role in the binding of L-Gln, negligible binding to either component alone | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | - |
reaction of ASI with chorismate and NH4+ | Pseudomonas putida |
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