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Literature summary for 4.1.3.27 extracted from

  • Widholm, J.M.
    Anthranilate synthetase from 5-methyltryptophan-susceptible and -resistant cultured Daucus carota cells (1972), Biochim. Biophys. Acta, 279, 48-57.
    View publication on PubMed
Search for more literature for 4.1.3.27

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Daucus carota
DL-4-methyltryptophan
-
 Daucus carota
DL-5-fluorotryptophan
-
 Daucus carota
DL-5-hydroxytryptophan
-
 Daucus carota
DL-5-Methyltryptophan 0.0054 mM, 50% inhibition Daucus carota
DL-6-Fluorotryptophan
-
 Daucus carota
Hg2+
-
 Daucus carota
L-Trp 0.0033 mM, 50% inhibition Daucus carota
Pb2+
-
 Daucus carota
PCMB
-
 Daucus carota
Zn2+
-
 Daucus carota

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
 chorismate wild-type enzyme Daucus carota
0.046
-
 chorismate mutant enzyme Daucus carota
0.237
-
 L-Gln mutant enzyme Daucus carota
0.249
-
 L-Gln wild-type enzyme Daucus carota

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can partially replace Mg2+ in activation Daucus carota
Mg2+ required Daucus carota
Mg2+ Km for the mutant enzyme: 0.036 mM, Km for the wild-type enzyme: 0.101 mM Daucus carota
Mn2+ can partially replace Mg2+ in activation Daucus carota

Organism

Organism UniProt Comment Textmining
Daucus carota
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:DL-5-methyltryptophan-grown cell normal cells and cells resistant to growth inhibition by DL-5-methyltryptophan Daucus carota
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chorismate + L-Gln
-
 Daucus carota anthranilate + pyruvate + L-glutamate
-
 ?
chorismate + NH4+ 10% of the activity with Gln Daucus carota ?
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0033
-
 L-Trp
-
 Daucus carota
0.0054
-
 DL-5-Methyltryptophan
-
 Daucus carota