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Literature summary for 4.1.2.4 extracted from

  • Kim, Y.M.; Choi, N.S.; Kim, Y.O.; Son, D.H.; Chang, Y.H.; Song, J.J.; Kim, J.S.
    Expression and characterization of a novel deoxyribose 5-phosphate aldolase from Paenibacillus sp. EA001 (2010), J. Microbiol. Biotechnol., 20, 995-1000.
    View publication on PubMed
Search for more literature for 4.1.2.4

Activating Compound

Activating Compound Comment Organism Structure
acetaldehyde the enzyme shows the highest activity at up to 100 mM acetaldehyde, and also shows some activity at 300 mM acetaldehyde Paenibacillus sp.

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Paenibacillus sp.

General Stability

General Stability Organism
the enzyme shows stability toward a high concentration of acetaldehyde (100 mM) Paenibacillus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
145
-
 2-deoxy-D-ribose 5-phosphate in 100 mM Tris-HCl buffer (pH 8.8), at 50°C Paenibacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24500
-
 x * 24500, calculated from amino acid sequence Paenibacillus sp.
24500
-
 x * 24500, estimated from SDS-PAGE Paenibacillus sp.

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. C7E719
-
-
Paenibacillus sp. EA001 C7E719
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Paenibacillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
44
-
 crude extract, using 2-deoxy-D-ribose 5-phosphate as substrate, in 100 mM Tris-HCl buffer (pH 8.8), at 50°C Paenibacillus sp.
62
-
 after 1.4fold purification, using 2-deoxy-D-ribose 5-phosphate as substrate, in 100 mM Tris-HCl buffer (pH 8.8), at 50°C Paenibacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-ribose 5-phosphate
-
 Paenibacillus sp. D-glyceraldehyde 3-phosphate + acetaldehyde
-
 r
2-deoxy-D-ribose 5-phosphate
-
 Paenibacillus sp. EA001 D-glyceraldehyde 3-phosphate + acetaldehyde
-
 r
D-glyceraldehyde 3-phosphate + acetaldehyde
-
 Paenibacillus sp. 2-deoxy-D-ribose 5-phosphate
-
 r
D-glyceraldehyde 3-phosphate + acetaldehyde
-
 Paenibacillus sp. EA001 2-deoxy-D-ribose 5-phosphate
-
 r

Subunits

Subunits Comment Organism
? x * 24500, calculated from amino acid sequence Paenibacillus sp.
? x * 24500, estimated from SDS-PAGE Paenibacillus sp.

Synonyms

Synonyms Comment Organism
DeoC
-
 Paenibacillus sp.
deoxyribose 5-phosphate aldolase
-
 Paenibacillus sp.
DERA
-
 Paenibacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Paenibacillus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 70
-
 Paenibacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 the enzyme retains full activity after 1 h at 35-55°C, the enzyme retains 80% activity after incubation at 60°C for 1 h, almost no activity is observed after 1 h at 65°C Paenibacillus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
320
-
 2-deoxy-D-ribose 5-phosphate in 100 mM Tris-HCl buffer (pH 8.8), at 50°C Paenibacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Paenibacillus sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 10
-
 Paenibacillus sp.