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Literature summary for 4.1.2.4 extracted from

  • Wang, Q.; Chen, R.; Du, P.; Wu, H.; Pei, X.; Yang, B.; Yang, L.; Huang, L.; Liu, J.; Xie, T.
    Cloning and characterization of thermostable-deoxy-D-ribose-5-phosphate aldolase from Hyperthermus butylicus (2010), Afr. J. Biotechnol., 9, 2898-2905.
No PubMed abstract available
Search for more literature for 4.1.2.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Hyperthermus butylicus

General Stability

General Stability Organism
the enzyme retains more than 75% activity after exposure for 8 h to 300 mM acetaldehyde at 25°C. Hyperthermus butylicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 2-deoxy-D-ribose 5-phosphate in 100 mM sodium acetate buffer (pH 5.5) at 25°C Hyperthermus butylicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 2 * 26000, SDS-PAGE Hyperthermus butylicus
26400
-
 2 * 26400, calculated from amino acid sequence Hyperthermus butylicus
52000
-
 gel filtration Hyperthermus butylicus

Organic Solvent Stability

Organic Solvent Comment Organism
1,4-dioxane the enzyme retains 28% and 78% activity in 20% (v/v) and 5% (v/v) 1,4-dioxane, respectively Hyperthermus butylicus
acetonitrile the enzyme loses activity completely in 20% (v/v) and shows 64% activity in 5% (v/v) acetonitrile Hyperthermus butylicus
dimethyl sulfoxide the enzyme retains 61% and 78% activity in 20% (v/v) and 5% (v/v) dimethylsulfoxide, respectively Hyperthermus butylicus
isopropanol the enzyme retains 11% and 72% activity in 20% (v/v) and 5% (v/v) isopropanol, respectively Hyperthermus butylicus
Methanol the enzyme retains 67% and 89% activity in 20% (v/v) and 5% (v/v) methanol, respectively Hyperthermus butylicus
tetrahydrofuran the enzyme loses activity completely in 20% (v/v) and shows 72% activity in 5% (v/v) tetrahydrofuran Hyperthermus butylicus

Organism

Organism UniProt Comment Textmining
Hyperthermus butylicus A2BLE9
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Hyperthermus butylicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.2
-
 crude cell extract, in 100 mM sodium acetate buffer (pH 5.5) at 25°C Hyperthermus butylicus
0.5
-
 after 2.5fold purification, in 100 mM sodium acetate buffer (pH 5.5) at 25°C Hyperthermus butylicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-ribose 5-phosphate
-
 Hyperthermus butylicus D-glyceraldehyde 3-phosphate + acetaldehyde
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 26000, SDS-PAGE Hyperthermus butylicus
homodimer 2 * 26400, calculated from amino acid sequence Hyperthermus butylicus

Synonyms

Synonyms Comment Organism
2-deoxy-D-ribose-5-phosphate aldolase
-
 Hyperthermus butylicus
DERA
-
 Hyperthermus butylicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Hyperthermus butylicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 80 the enzyme exhibits similar activity at temperatures between 70-80°C. The activity sharply decreases below 70°C and above 80°C Hyperthermus butylicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90 100 no activity loss is observed after incubation at 90°C for 10 min. After incubation at 100°C for 10 min, the enzyme retains 80% residual activity Hyperthermus butylicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Hyperthermus butylicus

pH Range

pH Minimum pH Maximum Comment Organism
5 6 more than 65% activity between pH 5.0 and 6.0, enzyme activity decreases sharply below pH 5.0 and above pH 7.0 Hyperthermus butylicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 11 after heating at pH levels ranging from 4.0 to 11.0 for 30 min at 50°C, the enzyme shows no loss of activity Hyperthermus butylicus