Literature summary for 4.1.2.4 extracted from

Pei, X.; Wang, Q.; Qiu, X.; Ying, L.; Tao, J.; Xie, T.
The fed-batch production of a thermophilic 2-deoxyribose-5-phosphate aldolase (DERA) in Escherichia coli by exponential feeding strategy control (2010), Appl. Biochem. Biotechnol., 162, 1423-1434.

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Application

Application	Comment	Organism
biotechnology	efficient process for the production of thermophilic DERA designed from the point of view of recombinant enzyme concentration and productivity, which is economically and technically viable and can be used as a guide for production of other synthetically useful enzymes	Hyperthermus butylicus

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 (DE3) from pET303-DERA008 in both batch and fed-batch cultures, under the control of the IPTG-inducible T7 promoter	Hyperthermus butylicus

Organism

Organism	UniProt	Comment	Textmining
Hyperthermus butylicus	-	-	-

Synonyms

Synonyms	Comment	Organism
2-Deoxyribose-5-phosphate aldolase	-	Hyperthermus butylicus
DERA	-	Hyperthermus butylicus

Expression

Organism	Comment	Expression
Hyperthermus butylicus	when the concentration of acetate is over 1.0 g/l, DERA expression level decreases	down
Hyperthermus butylicus	2 mmol IPTG induces the expression of DERA. As the induction proceeds, the target protein expression level first increases and then decreases, and DERA concentration improves and reaches a plateau after 6 h of induction. DERA concentration increases with the biomass growth and peaks at the end of the culture period at 5.12 g/l with total volumetric productivity of 0.256 gl-1h-1, which are over 10 and 5fold higher than conventional batch culture, respectively	up

General Information

General Information	Comment	Organism
physiological function	DERA catalyzes a sequential aldol reaction that is useful to synthesize a key fragment of atorvastatin	Hyperthermus butylicus

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Release 2023.1 (January 2023)