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Literature summary for 4.1.2.4 extracted from

  • Ueno, A.; Dautu, G.; Munyaka, B.; Carmen, G.; Kobayashi, Y.; Igarashi, M.
    Toxoplasma gondii: Identification and characterization of bradyzoite-specific deoxyribose phosphate aldolase-like gene (TgDPA) (2009), Exp. Parasitol., 121, 55-63.
    View publication on PubMed
Search for more literature for 4.1.2.4

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pGEX-6P-2 for expression in Escherichia coli BL21DE3 pLysS cells and into pcDNA6/V5-HisA for transfection of HEK-293T cells Toxoplasma gondii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 predicted Toxoplasma gondii
31000
-
 transfected HEK-293T cells, determined by SDS-PAGE and Western blot analysis Toxoplasma gondii
57000
-
 GST-fused protein expressed in Escherichia coli cells, determined by SDS-PAGE Toxoplasma gondii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-deoxy-D-ribose 5-phosphate Toxoplasma gondii
-
 D-glyceraldehyde 3-phosphate + acetaldehyde
-
 ?

Organism

Organism UniProt Comment Textmining
Toxoplasma gondii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
the GST-fused protein is affinity purified by glutathione-Sepharose beads Toxoplasma gondii

Source Tissue

Source Tissue Comment Organism Textmining
bradyzoite
-
 Toxoplasma gondii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-ribose 5-phosphate
-
 Toxoplasma gondii D-glyceraldehyde 3-phosphate + acetaldehyde
-
 ?

Synonyms

Synonyms Comment Organism
deoxyribose-phosphate aldolase
-
 Toxoplasma gondii
TgDPA
-
 Toxoplasma gondii