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Literature summary for 4.1.2.14 extracted from

  • Cheriyan, M.; Toone, E.J.; Fierke, C.A.
    Mutagenesis of the phosphate-binding pocket of KDPG aldolase enhances selectivity for hydrophobic substrates (2007), Protein Sci., 16, 2368-2377.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.2.14

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli K-12 cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
S184A the mutation decreases the efficiency of KDPG retro-aldol cleavage only modestly Escherichia coli
S184D the mutation leads to strongly reduced enzymatic activity Escherichia coli
S184L the mutation is located in the substrate-binding pocket, interacts with the phosphate moiety of KDPG, and improves the catalytic efficiency for the synthesis of a precursor for nikkomycin by 40fold Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate wild type enzyme, in 100 mM HEPES, pH 7.5 Escherichia coli
0.16
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate mutant enzyme S184A, in 100 mM HEPES, pH 7.5 Escherichia coli
0.35
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate mutant enzyme S184L, in 100 mM HEPES, pH 7.5 Escherichia coli
0.95
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate mutant enzyme S184L, in 100 mM HEPES, pH 7.5 Escherichia coli
1.2
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate mutant enzyme S184D, in 100 mM HEPES, pH 7.5 Escherichia coli
1.5
-
 2-dehydro-3-deoxy-D-gluconate mutant enzyme S184L, in 100 mM HEPES, pH 7.5 Escherichia coli
1.8
-
 2-dehydro-3-deoxy-D-gluconate mutant enzyme S184D, in 100 mM HEPES, pH 7.5 Escherichia coli
1.9
-
 2-dehydro-3-deoxy-D-gluconate wild type enzyme, in 100 mM HEPES, pH 7.5 Escherichia coli
2.3
-
 2-dehydro-3-deoxy-D-gluconate mutant enzyme S184A, in 100 mM HEPES, pH 7.5 Escherichia coli
3
-
 2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate mutant enzyme S184A, in 100 mM HEPES, pH 7.5 Escherichia coli
3
-
 2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate mutant enzyme S184D, in 100 mM HEPES, pH 7.5 Escherichia coli
3
-
 2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate mutant enzyme S184L, in 100 mM HEPES, pH 7.5 Escherichia coli
26
-
 2-dehydro-4-hydroxyoctonate mutant enzyme S184L, in 100 mM HEPES, pH 7.5 Escherichia coli
32
-
 2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate wild type enzyme, in 100 mM HEPES, pH 7.5 Escherichia coli
51
-
 2-dehydro-4-hydroxyoctonate mutant enzyme S184D, in 100 mM HEPES, pH 7.5 Escherichia coli
56
-
 2-dehydro-4-hydroxyoctonate mutant enzyme S184A, in 100 mM HEPES, pH 7.5 Escherichia coli
150
-
 2-dehydro-4-hydroxyoctonate wild type enzyme, in 100 mM HEPES, pH 7.5 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-charged chelating Sepharose Fast Flow column chromatography Escherichia coli

Storage Stability

Storage Stability Organism
4°C, HEPES (100 mM, pH 7.5), NaCl (100 mM), 10% glycerol, at concentrations ranging from 1 to 16 mM, up to 1 year, remains stable Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydro-3-deoxy-D-galactonate 6-phosphate
-
 Escherichia coli pyruvate + D-glyceraldehyde 3-phosphate
-
 ?
2-dehydro-3-deoxy-D-gluconate
-
 Escherichia coli ?
-
 ?
2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
 Escherichia coli pyruvate + D-glyceraldehyde 3-phosphate
-
 ?
2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate
-
 Escherichia coli ?
-
 ?
2-dehydro-4-hydroxyoctonate
-
 Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
2-keto-3-deoxy-6-phosphogluconate aldolase
-
 Escherichia coli
KDPG aldolase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00029
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate mutant enzyme S184D, in 100 mM HEPES, pH 7.5 Escherichia coli
0.0026
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate mutant enzyme S184L, in 100 mM HEPES, pH 7.5 Escherichia coli
0.0063
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate wild type enzyme, in 100 mM HEPES, pH 7.5 Escherichia coli
0.019
-
 2-dehydro-3-deoxy-D-gluconate wild type enzyme, in 100 mM HEPES, pH 7.5 Escherichia coli
0.04
-
 2-dehydro-3-deoxy-D-gluconate mutant enzyme S184A, in 100 mM HEPES, pH 7.5 Escherichia coli
0.08
-
 2-dehydro-3-deoxy-D-gluconate mutant enzyme S184L, in 100 mM HEPES, pH 7.5 Escherichia coli
0.11
-
 2-dehydro-3-deoxy-D-gluconate mutant enzyme S184D, in 100 mM HEPES, pH 7.5 Escherichia coli
1.8
-
 2-dehydro-4-hydroxyoctonate mutant enzyme S184D, in 100 mM HEPES, pH 7.5 Escherichia coli
2
-
 2-dehydro-4-hydroxyoctonate wild type enzyme, in 100 mM HEPES, pH 7.5 Escherichia coli
2.6
-
 2-dehydro-4-hydroxyoctonate mutant enzyme S184A, in 100 mM HEPES, pH 7.5 Escherichia coli
2.9
-
 2-dehydro-4-hydroxyoctonate mutant enzyme S184L, in 100 mM HEPES, pH 7.5 Escherichia coli
3.2
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate mutant enzyme S184D, in 100 mM HEPES, pH 7.5 Escherichia coli
6
-
 2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate mutant enzyme S184D, in 100 mM HEPES, pH 7.5 Escherichia coli
11
-
 2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate wild type enzyme, in 100 mM HEPES, pH 7.5 Escherichia coli
27
-
 2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate mutant enzyme S184A, in 100 mM HEPES, pH 7.5 Escherichia coli
40
-
 2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate mutant enzyme S184L, in 100 mM HEPES, pH 7.5 Escherichia coli
74
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate mutant enzyme S184L, in 100 mM HEPES, pH 7.5 Escherichia coli
83
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate wild type enzyme, in 100 mM HEPES, pH 7.5 Escherichia coli
97
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate mutant enzyme S184A, in 100 mM HEPES, pH 7.5 Escherichia coli