Cloned (Comment) | Organism |
---|---|
- |
Giardia intestinalis |
Crystallization (Comment) | Organism |
---|---|
D83A and D255A mutant enzymes, hanging drop vapor diffusion method, using 18-25% (w/v) glycol 3350 and 0.2 M NH4NO3 | Giardia intestinalis |
Protein Variants | Comment | Organism |
---|---|---|
D255A | the mutation results in an enzyme that possesses double specificity, now cleaving D-tagatose 1,6-bisphosphate (albeit with low efficacy) while maintaining activity toward D-fructose 1,6-bisphosphate at a 50fold lower catalytic efficacy compared with that of wild type FBPA | Giardia intestinalis |
D83A | inactive | Giardia intestinalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-tagatose 1,6-bisphosphate | competitive inhibitor | Giardia intestinalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
D-fructose 1,6-bisphosphate | wild type enzyme, in 50 mM K+-HEPES (pH 7.5), at 25°C | Giardia intestinalis | |
0.016 | - |
D-fructose 1,6-bisphosphate | mutant enzyme D255A, in 50 mM K+-HEPES (pH 7.5), at 25°C | Giardia intestinalis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | FBPA belongs to the class II zinc-dependent aldolase family, each subunit contains a Zn2+ cofactor | Giardia intestinalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Giardia intestinalis | O97447 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Giardia intestinalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | - |
Giardia intestinalis | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FBPA | - |
Giardia intestinalis |
fructose-1,6-bisphosphate aldolase | - |
Giardia intestinalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.63 | - |
D-fructose 1,6-bisphosphate | mutant enzyme D255A, in 50 mM K+-HEPES (pH 7.5), at 25°C | Giardia intestinalis | |
3.55 | - |
D-fructose 1,6-bisphosphate | wild type enzyme, in 50 mM K+-HEPES (pH 7.5), at 25°C | Giardia intestinalis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
40 | - |
D-fructose 1,6-bisphosphate | mutant enzyme D255A, in 50 mM K+-HEPES (pH 7.5), at 25°C | Giardia intestinalis | |
2000 | - |
D-fructose 1,6-bisphosphate | wild type enzyme, in 50 mM K+-HEPES (pH 7.5), at 25°C | Giardia intestinalis |