Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.2.13 extracted from

  • Galkin, A.; Li, Z.; Li, L.; Kulakova, L.; Pal, L.R.; Dunaway-Mariano, D.; Herzberg, O.
    Structural insights into the substrate binding and stereoselectivity of Giardia fructose-1,6-bisphosphate aldolase (2009), Biochemistry, 48, 3186-3196.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Giardia intestinalis

Crystallization (Commentary)

Crystallization (Comment) Organism
D83A and D255A mutant enzymes, hanging drop vapor diffusion method, using 18-25% (w/v) glycol 3350 and 0.2 M NH4NO3 Giardia intestinalis

Protein Variants

Protein Variants Comment Organism
D255A the mutation results in an enzyme that possesses double specificity, now cleaving D-tagatose 1,6-bisphosphate (albeit with low efficacy) while maintaining activity toward D-fructose 1,6-bisphosphate at a 50fold lower catalytic efficacy compared with that of wild type FBPA Giardia intestinalis
D83A inactive Giardia intestinalis

Inhibitors

Inhibitors Comment Organism Structure
D-tagatose 1,6-bisphosphate competitive inhibitor Giardia intestinalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0017
-
D-fructose 1,6-bisphosphate wild type enzyme, in 50 mM K+-HEPES (pH 7.5), at 25°C Giardia intestinalis
0.016
-
D-fructose 1,6-bisphosphate mutant enzyme D255A, in 50 mM K+-HEPES (pH 7.5), at 25°C Giardia intestinalis

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ FBPA belongs to the class II zinc-dependent aldolase family, each subunit contains a Zn2+ cofactor Giardia intestinalis

Organism

Organism UniProt Comment Textmining
Giardia intestinalis O97447
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Giardia intestinalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-bisphosphate
-
Giardia intestinalis glycerone phosphate + D-glyceraldehyde 3-phosphate
-
?

Synonyms

Synonyms Comment Organism
FBPA
-
Giardia intestinalis
fructose-1,6-bisphosphate aldolase
-
Giardia intestinalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.63
-
D-fructose 1,6-bisphosphate mutant enzyme D255A, in 50 mM K+-HEPES (pH 7.5), at 25°C Giardia intestinalis
3.55
-
D-fructose 1,6-bisphosphate wild type enzyme, in 50 mM K+-HEPES (pH 7.5), at 25°C Giardia intestinalis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
40
-
D-fructose 1,6-bisphosphate mutant enzyme D255A, in 50 mM K+-HEPES (pH 7.5), at 25°C Giardia intestinalis
2000
-
D-fructose 1,6-bisphosphate wild type enzyme, in 50 mM K+-HEPES (pH 7.5), at 25°C Giardia intestinalis