Literature summary for 4.1.2.13 extracted from

Galkin, A.; Kulakova, L.; Melamud, E.; Li, L.; Wu, C.; Mariano, P.; Dunaway-Mariano, D.; Nash, T.E.; Herzberg, O.
Characterization, kinetics, and crystal structures of fructose-1,6-bisphosphate aldolase from the human parasite, Giardia lamblia (2007), J. Biol. Chem., 282, 4859-4867.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain BL21(DE3)Star	Giardia intestinalis

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapour diffusion method with 18-23% polyethylene glycol monomethyl ether 2000, 0.1 M Tris-HCl (pH 8.8), and 0.2 M MgCl2	Giardia intestinalis

Protein Variants

Protein Variants	Comment	Organism
D83A	impaired enzyme activity	Giardia intestinalis

Inhibitors

Inhibitors	Comment	Organism	Structure
Phosphoglycolohydroxamate	-	Giardia intestinalis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0017	-	D-fructose 1,6-bisphosphate	at pH 7.5 and 25°C, in the absence of Zn2+	Giardia intestinalis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	dependent, the active site contains a zinc ion	Giardia intestinalis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
35380	-	calculated from amino acid sequence	Giardia intestinalis

Organism

Organism	UniProt	Comment	Textmining
Giardia intestinalis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Sepharose Q chromatography, Sephacryl 100 gel filtration, and phenyl-Sepharose chromatography	Giardia intestinalis

Source Tissue

Source Tissue	Comment	Organism	Textmining
trophozoite	-	Giardia intestinalis	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose 1,6-bisphosphate	-	Giardia intestinalis	glycerone phosphate + D-glyceraldehyde 3-phosphate	-	?
additional information	FBPA exhibits very low activity toward D-tagatose-1,6-bisphosphate	Giardia intestinalis	?	-	?

Subunits

Subunits	Comment	Organism
homodimer	x-ray crystallography	Giardia intestinalis

Synonyms

Synonyms	Comment	Organism
FBPA	-	Giardia intestinalis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.55	-	D-fructose 1,6-bisphosphate	at pH 7.5 and 25°C, in the absence of Zn2+	Giardia intestinalis

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0024	-	Phosphoglycolohydroxamate	at pH 7.5 and 25°C, in the absence of Zn2+	Giardia intestinalis

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Release 2023.1 (January 2023)