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Literature summary for 4.1.2.13 extracted from

  • Ramsaywak, P.C.; Labbe, G.; Siemann, S.; Dmitrienko, G.I.; Guillemette, J.G.
    Molecular cloning, expression, purification, and characterization of fructose 1,6-bisphosphate aldolase from Mycobacterium tuberculosis--a novel class II A tetramer (2004), Protein Expr. Purif., 37, 220-228.
    View publication on PubMed
Search for more literature for 4.1.2.13

Cloned(Commentary)

Cloned (Comment) Organism
subcloned in the Escherichia coli vector pT7-7. Attempts to express the enzyme with an N-terminal fusion tag yields inactive, mostly insoluble protein Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM, completely abolishes activity Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 D-fructose 1,6-bisphosphate pH 7.3, 28°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ the native recombinant enzyme is zinc-dependent, contains 0.5 Zn2+ per monomer Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
158000
-
 gel filtration Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WQA3 H37Rv
-
Mycobacterium tuberculosis H37Rv P9WQA3 H37Rv
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
35.1
-
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-bisphosphate
-
 Mycobacterium tuberculosis glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?
D-fructose 1,6-bisphosphate
-
 Mycobacterium tuberculosis H37Rv glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?

Subunits

Subunits Comment Organism
tetramer
-
 Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
class II FBP aldolase
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21
-
 D-fructose 1,6-bisphosphate pH 7.3, 28°C Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Mycobacterium tuberculosis

pH Range

pH Minimum pH Maximum Comment Organism
7.3 8.5 pH 7.3: about 70% of maximal activity, pH 8.5: about 55% of maximal activity Mycobacterium tuberculosis