Literature summary for 4.1.2.13 extracted from

Dax, C.; Coincon, M.; Sygusch, J.; Blonski, C.
Hydroxynaphthaldehyde phosphate derivatives as potent covalent Schiff base inhibitors of fructose-1,6-bisphosphate aldolase (2005), Biochemistry, 44, 5430-5443.

Search for more literature for 4.1.2.13

Protein Variants

Protein Variants	Comment	Organism
K107M	46fold increase in Ki-value for naphthyl 2,6-bisphosphate, 11.5fold increase in KM-value for D-fructose 1,6-diphosphate. The structure of the enzyme is isomorphous with respect to wild-type enzyme	Oryctolagus cuniculus
K146M	decrease in rate of complex formation between enzyme and inhibitor 1-hydroxy-2-naphthaldehyde 6-phosphate, but no change in ability to form the complex	Oryctolagus cuniculus
S271	mutation decreases by 1 order of magnitude the affinity of 1-hydroxy-2-naphthaldehyde 6-phosphate for the aldolase active site but does not modify its ability to catalyze Schiff base formation	Oryctolagus cuniculus

Inhibitors

Inhibitors	Comment	Organism	Structure
1-hydroxy-2-naphthaldehyde 6-phosphate	slow-binding	Oryctolagus cuniculus
naphthyl 2,6-bisphosphate	competitive	Oryctolagus cuniculus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.013	-	D-fructose 1,6-bisphosphate	wild-type enzyme	Oryctolagus cuniculus
0.15	-	D-fructose 1,6-bisphosphate	mutant enzyme K107M	Oryctolagus cuniculus

Organism

Organism	UniProt	Comment	Textmining
Oryctolagus cuniculus	P00883	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
muscle	-	Oryctolagus cuniculus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose 1,6-bisphosphate	-	Oryctolagus cuniculus	glycerone phosphate + D-glyceraldehyde 3-phosphate	-	?

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00028	-	naphthyl 2,6-bisphosphate	wild-type enzyme	Oryctolagus cuniculus
0.013	-	naphthyl 2,6-bisphosphate	mutant enzyme K107M	Oryctolagus cuniculus
0.125	-	1-hydroxy-2-naphthaldehyde 6-phosphate	wild-type enzyme	Oryctolagus cuniculus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)