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Literature summary for 4.1.2.13 extracted from

  • Chevalier, N.; Callens, M.; Michels, P.A.M.
    High-level expression of Trypanosoma brucei fructose 1,6-bisphosphate aldolase in Escherichia coli and purification of the enzyme (1995), Protein Expr. Purif., 6, 39-44.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0092
-
D-fructose 1,6-bisphosphate pH 7.6, native enzyme Trypanosoma brucei
0.0168
-
D-fructose 1,6-bisphosphate pH 7.6, recombinant enzyme Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
glycosome
-
Trypanosoma brucei 20015
-

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Trypanosoma brucei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-bisphosphate
-
Trypanosoma brucei glycerone phosphate + D-glyceraldehyde 3-phosphate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10.3
-
D-fructose 1,6-bisphosphate pH 7.6, native enzyme Trypanosoma brucei
10.9
-
D-fructose 1,6-bisphosphate pH 7.6, recombinant enzyme Trypanosoma brucei

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9.3 more than 50% of optimal activity in the range, recombinant enzyme Trypanosoma brucei

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.4 9.4 25°C, 22 h, enzyme concentration: 0.27 mg/ml, less than 30% loss of activity, recombinant enzyme Trypanosoma brucei