Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0092 | - |
D-fructose 1,6-bisphosphate | pH 7.6, native enzyme | Trypanosoma brucei | |
0.0168 | - |
D-fructose 1,6-bisphosphate | pH 7.6, recombinant enzyme | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
glycosome | - |
Trypanosoma brucei | 20015 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Trypanosoma brucei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | - |
Trypanosoma brucei | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.3 | - |
D-fructose 1,6-bisphosphate | pH 7.6, native enzyme | Trypanosoma brucei | |
10.9 | - |
D-fructose 1,6-bisphosphate | pH 7.6, recombinant enzyme | Trypanosoma brucei |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.3 | more than 50% of optimal activity in the range, recombinant enzyme | Trypanosoma brucei |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.4 | 9.4 | 25°C, 22 h, enzyme concentration: 0.27 mg/ml, less than 30% loss of activity, recombinant enzyme | Trypanosoma brucei |