General Stability | Organism |
---|---|
no inactivation by dialysis against 0.2 mM of either EDTA or o-phenanthroline in 0.05 M Tris-acetate, pH 7.5, containing 0.2 mM dithiothreitol, at 4°C, overnight | Comamonas testosteroni |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4,6-Trinitrobenzene sulfonate | inhibition is partially prevented by 4,5-dihydroxyphthalate or 4-hydroxyphthalate, 1 mM | Comamonas testosteroni | |
3-hydroxybenzoate | - |
Comamonas testosteroni | |
3-methylcatechol | weak | Comamonas testosteroni | |
4-hydroxybenzoate | - |
Comamonas testosteroni | |
AgCl | - |
Comamonas testosteroni | |
anthranilate | competitive | Comamonas testosteroni | |
benzoate | - |
Comamonas testosteroni | |
gentisate | weak | Comamonas testosteroni | |
HgCl2 | - |
Comamonas testosteroni | |
PCMB | - |
Comamonas testosteroni | |
Phthalate | - |
Comamonas testosteroni | |
protocatechuate | - |
Comamonas testosteroni | |
salicylate | - |
Comamonas testosteroni | |
terephthalate | competitive | Comamonas testosteroni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0105 | - |
4,5-Dihydroxyphthalate | - |
Comamonas testosteroni | |
1.25 | - |
4-Hydroxyphthalate | - |
Comamonas testosteroni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
4 * 38000, SDS-PAGE | Comamonas testosteroni |
150000 | - |
gel filtration | Comamonas testosteroni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | - |
- |
- |
Comamonas testosteroni NH1000 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Comamonas testosteroni |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
58.9 | - |
- |
Comamonas testosteroni |
Storage Stability | Organism |
---|---|
4°C, 0.05 M Tris-acetate, pH 7.5, 0.2 mM dithiothreitol, 50% loss of activity after 4 days | Comamonas testosteroni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4,5-Dihydroxyphthalate | - |
Comamonas testosteroni | 3,4-Dihydroxybenzoate + CO2 | - |
? | |
4,5-Dihydroxyphthalate | - |
Comamonas testosteroni NH1000 | 3,4-Dihydroxybenzoate + CO2 | - |
? | |
4-Hydroxyphthalate | - |
Comamonas testosteroni | 3-Hydroxybenzoate | - |
? | |
4-Hydroxyphthalate | - |
Comamonas testosteroni NH1000 | 3-Hydroxybenzoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 38000, SDS-PAGE | Comamonas testosteroni |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | - |
pH 7.5, 3.5 h, about 20% loss of activity | Comamonas testosteroni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Comamonas testosteroni |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
27°C, 3.5 h, about 20% loss of activity | Comamonas testosteroni |