Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain KK-8(pDM) | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0113 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | at 37°C and pH 8.0 | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
x * 31000, SDS-PAGE | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | Pseudomonas aeruginosa | - |
1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
TALON metal affinity resin column chromatography | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
Pseudomonas aeruginosa | 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
IGPS | - |
Pseudomonas aeruginosa |
indole-3-glycerol phosphate synthase | - |
Pseudomonas aeruginosa |
TrpC | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
the enzyme is about 2fold more active at 37°C than it is at 25°C | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.1 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | at 37°C and pH 8.0 | Pseudomonas aeruginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.2 | activity begins to decrease above pH 8.2 and is 70% of maximum at pH 9.0 | Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the fifth reaction in the synthesis of tryptophan from chorismate | Pseudomonas aeruginosa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
980 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | at 37°C and pH 8.0 | Pseudomonas aeruginosa |