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Literature summary for 4.1.1.48 extracted from
- Phosphoribosylanthranilate isomerase and indoleglycerol-phosphate synthase: tryptophan biosynthetic enzymes from Thermotoga maritima (2001), Methods Enzymol., 331, 270-280.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | - |
Thermotoga maritima |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000006 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 25°C | Thermotoga maritima | |
| 0.000014 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 45°C | Thermotoga maritima | |
| 0.000053 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 60°C | Thermotoga maritima | |
| 0.000123 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | pH 7.5, 80°C | Thermotoga maritima | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23600 | - |
gel filtration | Thermotoga maritima |
| 28700 | - |
1 * 28700, deduced from gene sequence | Thermotoga maritima |
| 31000 | - |
equilibrium sedimentation analysis | Thermotoga maritima |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | - |
recombinant enzyme, expression in Escherichia coli | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.3 | - |
- |
Thermotoga maritima |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 1 mM potassium phosphate buffer pH 7.5, 1 mM dithiothreitol, stable for hours | Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | - |
Thermotoga maritima | 1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 28700, deduced from gene sequence | Thermotoga maritima |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 84 | - |
110 min, half-life for irreversible thermal denaturation | Thermotoga maritima |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Thermotoga maritima |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.27 | - |
phosphate | 60°C | Thermotoga maritima | |
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