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Literature summary for 4.1.1.48 extracted from

  • Hennig, M.; Darimont, B.; Sterner, R.; Kirschner, K.; Jansonius, J.N.
    2.0 A structure of indole-3-glycerol phosphate synthase from the hyperthermophile Sulfolobus solfataricus: possible determinants of protein stability (1995), Structure, 3, 1295-1306.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Saccharolobus solfataricus

Crystallization (Commentary)

Crystallization (Comment) Organism
2.0 A structure Saccharolobus solfataricus
vapour diffusion method in hanging drops, the 2.0 A crystal structure is determined and compared with the known 2.0 A structure of the IGPS domain of the bifunctional enzyme from Escherichia coli. Both enzymes have only 30% sequence identity, but share high structural similarity Saccharolobus solfataricus

General Stability

General Stability Organism
the higher stability of the enzyme from Sulfolobus solfataricus compared with the enzyme from E. coli seems to be the result of several improved interactions. Including a large number of salt bridges, stabilization of alpha-helices and strengthening of both polypeptide chain termini and solvent-exposed loops Escherichia coli
the higher stability of the enzyme from Sulfolobus solfataricus compared with the enzyme from E. coli seems to be the result of several improved interactions. Including a large number of salt bridges, stabilization of alpha-helices and strengthening of both polypeptide chain termini and solvent-exposed loops Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 25°C Saccharolobus solfataricus
0.09
-
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 25°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28600
-
x * 28600, SDS-PAGE Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus Q06121
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
Escherichia coli 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O
-
?
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
Saccharolobus solfataricus 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O
-
?
1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate
-
Escherichia coli 1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O
-
?
1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate
-
Saccharolobus solfataricus 1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O
-
?

Subunits

Subunits Comment Organism
? x * 28600, SDS-PAGE Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
eIGPS
-
Escherichia coli
indole-3-glycerol phosphate synthase
-
Escherichia coli
indole-3-glycerol phosphate synthase
-
Saccharolobus solfataricus
sIGPS
-
Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Escherichia coli
25
-
assay at Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
the higher stability of the indole-3-glycerol phosphate synthase from Sulfolobus solfataricus compared to indole-3-glycerol phosphate synthase from Escherichia coli seems to be the result of several improved interactions. These include a larger number of salt bridges, stabilization of alpha helices and strengthening of both polypeptide chain termini and solvent-exposed loops Escherichia coli
additional information
-
the higher stability of the indole-3-glycerol phosphate synthase from Sulfolobus solfataricus compared to indole-3-glycerol phosphate synthase from Escherichia coli seems to be the result of several improved interactions. These include a larger number of salt bridges, stabilization of alpha helices and strengthening of both polypeptide chain termini and solvent-exposed loops Saccharolobus solfataricus
44
-
half-life: 1.8 min Escherichia coli
89
-
half-life: 4.4 min Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.033
-
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 25°C Saccharolobus solfataricus
1.2
-
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 25°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia coli
7.5
-
assay at Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.36
-
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 25°C Saccharolobus solfataricus
13.3
-
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate pH 7.5, 25°C Escherichia coli