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Literature summary for 4.1.1.39 extracted from
- The evolution of RuBisCO stability at the thermal limit of photoautotrophy (2013), Mol. Biol. Evol., 30, 752-760.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Synechococcus sp. |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F15L | the mutant shows reduced carboxylation activity compared to the wild type | Synechococcus sp. |
| L138V | the mutant shows strongly reduced carboxylation activity compared to the wild type | Synechococcus sp. |
| L27I | the mutant shows about wild type carboxylation activity | Synechococcus sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-ribulose 1,5-bisphosphate + CO2 + H2O | Synechococcus sp. | - |
3-phospho-D-glycerate + H+ | - |
? |  |
| D-ribulose 1,5-bisphosphate + CO2 + H2O | Synechococcus sp. OH28 | - |
3-phospho-D-glycerate + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Synechococcus sp. | - |
- |
- |
| Synechococcus sp. OH28 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| FPLC 15 Q column chromatography and Superose 12 gel filtration | Synechococcus sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-ribulose 1,5-bisphosphate + CO2 + H2O | - |
Synechococcus sp. | 3-phospho-D-glycerate + H+ | - |
? | |
| D-ribulose 1,5-bisphosphate + CO2 + H2O | - |
Synechococcus sp. OH28 | 3-phospho-D-glycerate + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterohexadecamer | - |
Synechococcus sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ribulose-1, 5-bisphosphate carboxylase/oxygenase | - |
Synechococcus sp. |
| Rubisco | - |
Synechococcus sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 7 | 9.5 | the melting temperature is around 79.5°C | Synechococcus sp. |
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Release 2023.1 (January 2023)
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