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Literature summary for 4.1.1.39 extracted from

  • Mueller-Cajar, O.; Morell, M.; Whitney, S.M.
    Directed evolution of Rubisco in Escherichia coli reveals a specificity-determining hydrogen bond in the form II enzyme (2007), Biochemistry, 46, 14067-14074.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli XL1-Blue cells Rhodospirillum rubrum

Protein Variants

Protein Variants Comment Organism
D117H 40% reduced CO2/O2 specificity Rhodospirillum rubrum
D117V 40% reduced CO2/O2 specificity Rhodospirillum rubrum
H44N 40% reduced CO2/O2 specificity Rhodospirillum rubrum
H44Q 40% reduced CO2/O2 specificity Rhodospirillum rubrum
K191M inactive Rhodospirillum rubrum

Inhibitors

Inhibitors Comment Organism Structure
D-fructose-1,6-bisphosphate
-
Rhodospirillum rubrum
O2
-
Rhodospirillum rubrum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.149
-
CO2 wild type enzyme, at 25 °C, pH 8 Rhodospirillum rubrum
0.192
-
CO2 mutant enzyme D117H, at 25 °C, pH 8 Rhodospirillum rubrum
0.196
-
CO2 mutant enzyme D117V, at 25 °C, pH 8 Rhodospirillum rubrum
0.204
-
CO2 mutant enzyme H44N, at 25 °C, pH 8 Rhodospirillum rubrum
0.301
-
CO2 mutant enzyme H44Q, at 25 °C, pH 8 Rhodospirillum rubrum

Organism

Organism UniProt Comment Textmining
Rhodospirillum rubrum P04718
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rhodospirillum rubrum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribulose 1,5-bisphosphate + CO2 + H2O
-
Rhodospirillum rubrum 3-phospho-D-glycerate
-
?
D-ribulose 1,5-bisphosphate + O2
-
Rhodospirillum rubrum 3-phospho-D-glycerate + 2-phosphoglycolate
-
?

Subunits

Subunits Comment Organism
dimer
-
Rhodospirillum rubrum

Synonyms

Synonyms Comment Organism
Form II Rubisco dimeric form Rhodospirillum rubrum
ribulose-1,5-bisphosphate carboxylase/oxygenase
-
Rhodospirillum rubrum
Rubisco
-
Rhodospirillum rubrum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
O2 mutant enzyme H44N, at 25°C, pH 8 Rhodospirillum rubrum
1.1
-
O2 mutant enzyme D117H, at 25°C, pH 8 Rhodospirillum rubrum
1.2
-
O2 mutant enzyme H44Q, at 25°C, pH 8 Rhodospirillum rubrum
1.4
-
O2 wild type enzyme, at 25°C, pH 8 Rhodospirillum rubrum
1.5
-
O2 mutant enzyme D117V, at 25°C, pH 8 Rhodospirillum rubrum
7.4
-
CO2 mutant enzyme D117V, at 25°C, pH 8 Rhodospirillum rubrum
7.5
-
CO2 mutant enzyme D117H, at 25°C, pH 8 Rhodospirillum rubrum
9.3
-
CO2 mutant enzyme H44Q, at 25°C, pH 8 Rhodospirillum rubrum
9.8
-
CO2 mutant enzyme H44N, at 25°C, pH 8 Rhodospirillum rubrum
12.3
-
CO2 wild type enzyme, at 25°C, pH 8 Rhodospirillum rubrum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.116
-
O2 mutant enzyme H44N, at 25°C, pH 8 Rhodospirillum rubrum
0.153
-
O2 mutant enzyme D117H, at 25°C, pH 8 Rhodospirillum rubrum
0.159
-
O2 wild type enzyme, at 25°C, pH 8 Rhodospirillum rubrum
0.185
-
O2 mutant enzyme H44Q, at 25°C, pH 8 Rhodospirillum rubrum
0.199
-
O2 mutant enzyme D117V, at 25°C, pH 8 Rhodospirillum rubrum
0.245
-
D-fructose-1,6-bisphosphate mutant enzyme D117H, at 25°C, pH 8 Rhodospirillum rubrum
0.32
-
D-fructose-1,6-bisphosphate mutant enzyme H44N, at 25°C, pH 8 Rhodospirillum rubrum
0.347
-
D-fructose-1,6-bisphosphate mutant enzyme D117V, at 25°C, pH 8 Rhodospirillum rubrum
0.348
-
D-fructose-1,6-bisphosphate mutant enzyme H44Q, at 25°C, pH 8 Rhodospirillum rubrum
0.465
-
D-fructose-1,6-bisphosphate wild type enzyme, at 25°C, pH 8 Rhodospirillum rubrum