Protein Variants | Comment | Organism |
---|---|---|
C58S | site-directed mutagenesis, elimination of the chemically active site without any loss of activity | Rhodospirillum rubrum |
K166C | site-directed mutagenesis, able to process RuBP, but the major product is DiMP derived from beta-elimination of phosphate from the enediol | Rhodospirillum rubrum |
K166C/C58S | site-directed mutagenesis, virtually inactive double mutant | Rhodospirillum rubrum |
K166G | site-directed mutagenesis, able to process RuBP, but the major product is DiMP derived from beta-elimination of phosphate from the enediol | Rhodospirillum rubrum |
K166S | site-directed mutagenesis, able to process RuBP, but the major product is DiMP derived from beta-elimination of phosphate from the enediol | Rhodospirillum rubrum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 1,5-bisphosphate + CO2 + H2O | Rhodospirillum rubrum | rate-limiting reaction of overall photosynthetic CO2 assimilation and photorespiratory carbon oxidation | 3-phospho-D-glycerate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodospirillum rubrum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
mutant rbc genes transferred into pFL260 for expression in Escherichia coli MV1190 | Rhodospirillum rubrum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 1,5-bisphosphate + CO2 + H2O | - |
Rhodospirillum rubrum | 3-phospho-D-glycerate | - |
? | |
D-ribulose 1,5-bisphosphate + CO2 + H2O | rate-limiting reaction of overall photosynthetic CO2 assimilation and photorespiratory carbon oxidation | Rhodospirillum rubrum | 3-phospho-D-glycerate | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
ribulose-bisphosphate carboxylase/oxygenase | - |
Rhodospirillum rubrum |
Rubisco | - |
Rhodospirillum rubrum |