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Literature summary for 4.1.1.31 extracted from
- The bacterial-type phosphoenolpyruvate carboxylase isozyme from developing castor oil seeds is subject to in vivo regulatory phosphorylation at serine-451 (2012), FEBS Lett., 586, 1049-1054.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Ricinus communis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S425A | the mutant shows strongly increased Km values compared to the wild type enzyme | Ricinus communis |
| S425A/S451D | the mutant shows strongly increased Km values compared to the wild type enzyme | Ricinus communis |
| S451D | the mutant shows strongly increased Km values compared to the wild type enzyme | Ricinus communis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.74 | - |
phosphoenolpyruvate | wild type enzyme, at pH 7.3 and 25°C | Ricinus communis |  |
| 0.76 | - |
phosphoenolpyruvate | wild type enzyme, at pH 8.0 and 25°C | Ricinus communis | |
| 1 | - |
phosphoenolpyruvate | mutant enzyme S425A/S451D, at pH 7.3 and 25°C | Ricinus communis | |
| 1.6 | - |
phosphoenolpyruvate | mutant enzyme S425A/S451D, at pH 8.0 and 25°C | Ricinus communis | |
| 2 | - |
phosphoenolpyruvate | mutant enzyme S425A, at pH 7.3 and 25°C | Ricinus communis | |
| 2.1 | - |
phosphoenolpyruvate | mutant enzyme S451D, at pH 7.3 and 25°C | Ricinus communis | |
| 2.4 | - |
phosphoenolpyruvate | mutant enzyme S451D, at pH 8.0 and 25°C | Ricinus communis | |
| 2.7 | - |
phosphoenolpyruvate | mutant enzyme S425A, at pH 8.0 and 25°C | Ricinus communis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 900000 | - |
gel filtration | Ricinus communis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + HCO3- | Ricinus communis | - |
phosphate + oxaloacetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ricinus communis | - |
cultivar Baker 296 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | the enzyme is in vivo phosphorylated at Ser451 | Ricinus communis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+ affinity resin column chromatography, Superdex 200 gel filtration, and Superose-6 gel filtration | Ricinus communis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Ricinus communis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + HCO3- | - |
Ricinus communis | phosphate + oxaloacetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterooctamer | - |
Ricinus communis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PEPC | - |
Ricinus communis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Ricinus communis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | - |
Ricinus communis | |
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Release 2023.1 (January 2023)
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