Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Ricinus communis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-fructose 6-phosphate | 99% residual activity at 2 mM | Ricinus communis | |
D-glucose 1-phosphate | 95% residual activity at 2 mM | Ricinus communis | |
D-glucose 6-phosphate | 93% residual activity at 2 mM | Ricinus communis | |
glycerol 3-phosphate | 98% residual activity at 2 mM | Ricinus communis | |
L-aspartate | 98% residual activity at 2 mM | Ricinus communis | |
L-malate | 84% residual activity at 2 mM | Ricinus communis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.84 | - |
phosphoenolpyruvate | at pH 7.3 | Ricinus communis | |
0.87 | - |
phosphoenolpyruvate | at pH 8.0 | Ricinus communis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolutely dependent on, PPC4 exhibits a 4fold higher specific activity with saturating (10mM) Mg2+ relative to Mn2+ | Ricinus communis | |
Mn2+ | may replace Mg2+ | Ricinus communis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
118000 | - |
PPC4, gel filtration | Ricinus communis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Ricinus communis | recombinant PPC4 forms class-2 PEPC when combined with class-1 PEPCs | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ricinus communis | A6YM33 | PPC4 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, Superose-6 gel filtration, and Superdex 200 gel filtration | Ricinus communis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Ricinus communis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.3 | - |
clarified extract | Ricinus communis |
25.2 | - |
after 152fold purification | Ricinus communis |
Storage Stability | Organism |
---|---|
-20°C, 50% (v/v) glycerol, the final PPC4 preparation proves difficult to store as it slowly loses activity and is completely inactivated when rapidly thawed after freezing in liquid N2 | Ricinus communis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | recombinant PPC4 forms class-2 PEPC when combined with class-1 PEPCs | Ricinus communis | ? | - |
? | |
phosphoenolpyruvate + CO2 | - |
Ricinus communis | phosphate + oxaloacetate | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
bacterial-type PEPC | - |
Ricinus communis |
BTPC | - |
Ricinus communis |
PEPC | - |
Ricinus communis |
Phosphoenolpyruvate carboxylase | - |
Ricinus communis |
PPC | - |
Ricinus communis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Ricinus communis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
11 | - |
at pH 7.0 | Ricinus communis | L-malate | |
33 | - |
at pH 7.0 | Ricinus communis | L-aspartate |