Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glucose 6-phosphate | - |
Zea mays | |
glycine | - |
Zea mays |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3)pLysS | Zea mays |
Protein Variants | Comment | Organism |
---|---|---|
D228N | reduced apparent affinity for the activator glycine | Zea mays |
E229A | maximal activation caused by glycine is greatly reduced, significantly lowered sensitivity to the inhibitors malate and aspartate. K(0.5) for phosphoenolpyruvate is lower than wild-type value | Zea mays |
R226Q | maximal activation caused by glycine is greatly reduced, significantly lowered sensitivity to the inhibitors malate and aspartate. K(0.5) for phosphoenolpyruvate is significantly higher than that of wild-type enzyme | Zea mays |
R231A | decreased apparent affinity for the activator glucose 6-phosphate | Zea mays |
R232Q | decreased apparent affinity for the activator glucose 6-phosphate, reduced apparent affinity for the activator glycine | Zea mays |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aspartate | - |
Zea mays | |
malate | - |
Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + HCO3- | - |
Zea mays | phosphate + oxaloacetate | - |
? |