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Literature summary for 4.1.1.31 extracted from
- Purification and characterization of recombinant phosphoenolpyruvate carboxylase of Thermus sp. (1996), J. Biochem., 120, 518-524.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thermus sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Thermus sp. |  |
| Asp | - |
Thermus sp. | |
| ATP | - |
Thermus sp. | |
| fructose 1,6-diphosphate | - |
Thermus sp. | |
| glucose 6-phosphate | - |
Thermus sp. | |
| GTP | - |
Thermus sp. | |
| guanidine hydrochloride | - |
Thermus sp. | |
| malate | - |
Thermus sp. | |
| phosphate | - |
Thermus sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | - |
4 * 100000, SDS-PAGE | Thermus sp. |
| 400000 | - |
gel filtration | Thermus sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermus sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 29.4 | - |
- |
Thermus sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + CO2 | - |
Thermus sp. | phosphate + oxaloacetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 100000, SDS-PAGE | Thermus sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
in presence of 0.3 M acetyl-CoA and 2 mM phosphoenolpyruvate | Thermus sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 80 | about 50% of maximal activity at 50°C and 80°C | Thermus sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
half-life: 85 min | Thermus sp. |
| 90 | - |
half-life: 110 min | Thermus sp. |
| 95 | - |
half-life: 50 min | Thermus sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
at 30°C | Thermus sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Thermus sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | strongly dependent on the allosteric activator | Thermus sp. | |
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