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Literature summary for 4.1.1.23 extracted from

  • Goryanova, B.; Goldman, L.M.; Amyes, T.L.; Gerlt, J.A.; Richard, J.P.
    Role of a guanidinium cation-phosphodianion pair in stabilizing the vinyl carbanion intermediate of orotidine 5-phosphate decarboxylase-catalyzed reactions (2013), Biochemistry, 52, 7500-7511.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.23

Cloned(Commentary)

Cloned (Comment) Organism
-
 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
R235A mutation results in 12000fold decrease in catalytic efficiency with substrate orotidine 5'-phosphate and 75fold decrease with substrate 5-fluoroorotidine 5'-phosphate. The effect of the R235A mutation on the enzyme-catalyzed deuterium exchange is expressed predominantly as a change in the turnover number kex, whereas the effect on the enzyme-catalyzed decarboxylation of orotidine 5'-phosphate is expressed predominantly as a change in the Michaelis constant Km Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
 5-Fluoroorotidine 5'-phosphate wild-type, pH 7.1, 25°C Saccharomyces cerevisiae
0.0014
-
 orotidine 5'-phosphate wild-type, pH 7.1, 25°C Saccharomyces cerevisiae
0.058
-
 5-Fluoroorotidine 5'-phosphate mutant R235A, pH 7.1, 25°C Saccharomyces cerevisiae
1.4
-
 orotidine 5'-phosphate mutant R235A, pH 7.1, 25°C Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-Fluoroorotidine 5'-phosphate
-
 Saccharomyces cerevisiae 5-Fluorouridine 5'-phosphate + CO2
-
 ?
Orotidine 5'-phosphate
-
 Saccharomyces cerevisiae UMP + CO2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
 orotidine 5'-phosphate mutant R235A, pH 7.1, 25°C, presence of 0.1 M NCl Saccharomyces cerevisiae
15
-
 orotidine 5'-phosphate wild-type, pH 7.1, 25°C, presence of 0.1 M NaCl Saccharomyces cerevisiae
92
-
 5-Fluoroorotidine 5'-phosphate mutant R235A, pH 7.1, 25°C, presence of 0.1 M NaCl Saccharomyces cerevisiae
95
-
 5-Fluoroorotidine 5'-phosphate wild-type, pH 7.1, 25°C, presence of 0.1 M NaCl Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.91
-
 orotidine 5'-phosphate mutant R235A, pH 7.1, 25°C Saccharomyces cerevisiae
160
-
 5-Fluoroorotidine 5'-phosphate mutant R235A, pH 7.1, 25°C Saccharomyces cerevisiae
11000
-
 orotidine 5'-phosphate wild-type, pH 7.1, 25°C Saccharomyces cerevisiae
12000
-
 5-Fluoroorotidine 5'-phosphate wild-type, pH 7.1, 25°C Saccharomyces cerevisiae