Literature summary for 4.1.1.23 extracted from

Barnett, S.A.; Amyes, T.L.; Wood, B.M.; Gerlt, J.A.; Richard, J.P.
Dissecting the total transition state stabilization provided by amino acid side chains at orotidine 5-monophosphate decarboxylase: a two-part substrate approach (2008), Biochemistry, 47, 7785-7787.

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Protein Variants

Protein Variants	Comment	Organism
Q215A	mutant, effect of the mutation of the kinetic parameters for decarboxylation is determined	Saccharomyces cerevisiae
S154A	mutant, effect of the mutation of the kinetic parameters for decarboxylation is determined	Saccharomyces cerevisiae
S154A/Q215A	mutant, effect of the mutation of the kinetic parameters for decarboxylation is determined	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0016	-	orotidine 5'-monophosphate	wild type enzyme	Saccharomyces cerevisiae
0.05	-	orotidine 5'-monophosphate	Q215A mutant	Saccharomyces cerevisiae
0.11	-	orotidine 5'-monophosphate	S154/Q215A mutant	Saccharomyces cerevisiae
0.13	-	orotidine 5'-monophosphate	S154 mutant	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
Orotidine 5'-phosphate	Saccharomyces cerevisiae	-	UMP + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	P03962	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Orotidine 5'-phosphate	-	Saccharomyces cerevisiae	UMP + CO2	-	?

Synonyms

Synonyms	Comment	Organism
Orotidine 5'-monophosphate decarboxylase	-	Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	activity assay	Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.042	-	orotidine 5'-monophosphate	S154/Q215A mutant	Saccharomyces cerevisiae
0.082	-	orotidine 5'-monophosphate	S154 mutant	Saccharomyces cerevisiae
15	-	orotidine 5'-monophosphate	wild type enzyme	Saccharomyces cerevisiae
21	-	orotidine 5'-monophosphate	Q215A mutant	Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.1	-	activity assay	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)