Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methanothermobacter thermautotrophicus |
Crystallization (Comment) | Organism |
---|---|
ligand-free ODCase form and complexed with 6-azauridine 5-phosphate | Methanothermobacter thermautotrophicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-azauridine 5'-phosphate | mode of binding | Methanothermobacter thermautotrophicus | |
UMP | weak inhibition | Methanothermobacter thermautotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Orotidine 5'-phosphate | Methanothermobacter thermautotrophicus | last step in the biosynthesis of pyrimidine nucleotides | UMP + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Methanothermobacter thermautotrophicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
orotidine 5'-phosphate = UMP + CO2 | catalytic mechanism | Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Orotidine 5'-phosphate | catalytic mechanism, the remarkable catalytic power is almost exclusively achieved via ground state destabilization of the reactive part of substrate, which is compensated for by strong binding of the phosphate and ribose groups, and to a lesser extend via transition state stabilization, enzyme/active site structure, mode of substrate binding | Methanothermobacter thermautotrophicus | UMP + CO2 | - |
? | |
Orotidine 5'-phosphate | last step in the biosynthesis of pyrimidine nucleotides | Methanothermobacter thermautotrophicus | UMP + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Methanothermobacter thermautotrophicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | remarkable catalytic power | Methanothermobacter thermautotrophicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.46 | - |
UMP | - |
Methanothermobacter thermautotrophicus |