Cloned (Comment) | Organism |
---|---|
ura3 gene expression in Escherichia coli SS6130, expression in yeast | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-thiocarboxamidouridine 5'-phosphate | competitive, very strong, reversible inhibition | Saccharomyces cerevisiae | |
additional information | not inhibited by 6-carboxamidouridine 5-phosphate, recombinant ODCase expressed in Escherichia coli is not inhibited by 1,3-dimercaptopropanol and EDTA | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
orotidine 5'-phosphate | 22-25°C, pH 7.2, recombinant ODCase expressed in Escherichia coli | Saccharomyces cerevisiae | |
0.0007 | - |
orotidine 5'-phosphate | 22°C, native ODCase expressed in yeast | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | zinc-free fully active recombinant ODCase expressed in Escherichia coli, metals do not directly participate in the decarboxylation mechanism | Saccharomyces cerevisiae | |
Zn2+ | native ODCase expressed in yeast contains zinc, but not recombinant ODCase expressed in Escherichia coli, zinc is not involved in substrate decarboxylation | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
x * 27000, about, recombinant ODCase expressed in Escherichia coli, SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Orotidine 5'-phosphate | Saccharomyces cerevisiae | production of UMP, the biosynthetic precursor of pyrimidine nucleotides | UMP + CO2 | - |
? | |
Orotidine 5'-phosphate | Saccharomyces cerevisiae BJ5424 | production of UMP, the biosynthetic precursor of pyrimidine nucleotides | UMP + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae BJ5424 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | no posttranslational modifications in recombinant ODCase expressed in Escherichia coli, but presumably in native ODCase from yeast, in which the N-terminus is blocked | Saccharomyces cerevisiae |
Purification (Comment) | Organism |
---|---|
recombinant ODCase expressed in Escherichia coli | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
27.2 | - |
22°C, native ODCase expressed in yeast | Saccharomyces cerevisiae |
39.3 | - |
22-25°C, pH 7.2, recombinant ODCase expressed in Escherichia coli | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Orotidine 5'-phosphate | - |
Saccharomyces cerevisiae | UMP + CO2 | - |
? | |
Orotidine 5'-phosphate | production of UMP, the biosynthetic precursor of pyrimidine nucleotides | Saccharomyces cerevisiae | UMP + CO2 | - |
? | |
Orotidine 5'-phosphate | - |
Saccharomyces cerevisiae BJ5424 | UMP + CO2 | - |
? | |
Orotidine 5'-phosphate | production of UMP, the biosynthetic precursor of pyrimidine nucleotides | Saccharomyces cerevisiae BJ5424 | UMP + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27000, about, recombinant ODCase expressed in Escherichia coli, SDS-PAGE | Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the recombinant zinc-free ODCase expressed in Escherichia coli is more thermolabile than the zinc-containing ODCase expressed in yeast | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13 | - |
orotidine 5'-phosphate | 22°C, native ODCase expressed in yeast | Saccharomyces cerevisiae | |
19 | - |
orotidine 5'-phosphate | 22-25°C, pH 7.2, recombinant ODCase expressed in Escherichia coli | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000035 | - |
6-thiocarboxamidouridine 5'-phosphate | recombinant ODCase expressed in Escherichia coli | Saccharomyces cerevisiae |