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Literature summary for 4.1.1.23 extracted from
- Orotidine-5'-monophate decarboxylase from Pseudomonas aeruginosa PAO1: cloning, overexpression, and enzyme characterization (1994), Curr. Microbiol., 29, 353-359.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli and Pseudomonas aeruginosa pyrF mutant | Pseudomonas aeruginosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00991 | - |
orotidine 5'-phosphate | - |
Pseudomonas aeruginosa |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
non-denaturing PAGE | Pseudomonas aeruginosa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Orotidine 5'-phosphate | Pseudomonas aeruginosa | final step in pyrimidine biosynthesis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
PAO1 | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stable for 1 week | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Orotidine 5'-phosphate | - |
Pseudomonas aeruginosa | UMP + CO2 | - |
? | |
| Orotidine 5'-phosphate | final step in pyrimidine biosynthesis | Pseudomonas aeruginosa | ? | - |
? | |
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