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Literature summary for 4.1.1.21 extracted from

  • Tranchimand, S.; Starks, C.M.; Mathews, I.I.; Hockings, S.C.; Kappock, T.J.
    Treponema denticola PurE is a bacterial AIR carboxylase (2011), Biochemistry, 50, 4623-4637.
    View publication on PubMed
Search for more literature for 4.1.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Treponema denticola

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 14-16% (w/v) PEG 1000, 100 mM MgCl2, and 100 mM imidazole (pH 8.0) Treponema denticola

Protein Variants

Protein Variants Comment Organism
T40N the specific actzivity of the mutant is 20000fold lower than that of wild type enzyme Treponema denticola

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0038
-
 4-carboxy-5-aminoimidazole ribonucleotide 50 mM Tris-HCl (pH 8.0), at 10°C Treponema denticola
0.009
-
 4-carboxy-5-aminoimidazole ribonucleotide 50 mM Tris-HCl (pH 8.0), at 30°C Treponema denticola
0.06
-
 5-aminoimidazole ribonucleotide 50 mM Tris-HCl (pH 8.0), at 10°C Treponema denticola
13
-
 CO2 50 mM Tris-HCl (pH 8.0), at 10°C Treponema denticola

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17189
-
 8 * 17189, calculated from amino acid sequence Treponema denticola
17195
-
 8 * 17195, ESI-MS Treponema denticola
184000
-
 gel filtration Treponema denticola

Organism

Organism UniProt Comment Textmining
Treponema denticola Q73PV9
-
-

Purification (Commentary)

Purification (Comment) Organism
streptomycin precipitation, ammonium sulfate precipitation, phenyl Sepharose 6 column chromatography, hydroxyapatite column chromatography, and Superdex 200 gel filtration Treponema denticola

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-carboxy-5-aminoimidazole ribonucleotide + H+
-
 Treponema denticola 5-aminoimidazole ribonucleotide + CO2
-
 r
5-aminoimidazole ribonucleotide + CO2
-
 Treponema denticola 4-carboxy-5-aminoimidazole ribonucleotide + H+
-
 r
additional information the enzyme does not bind N5-carboxy-4-carboxy-5-aminoimidazole ribonucleotide Treponema denticola ?
-
 ?

Subunits

Subunits Comment Organism
homooctamer 8 * 17189, calculated from amino acid sequence Treponema denticola
homooctamer 8 * 17195, ESI-MS Treponema denticola

Synonyms

Synonyms Comment Organism
AIR carboxylase
-
 Treponema denticola
class II PurE
-
 Treponema denticola
PurE
-
 Treponema denticola

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
16
-
 4-carboxy-5-aminoimidazole ribonucleotide 50 mM Tris-HCl (pH 8.0), at 10°C Treponema denticola
65
-
 4-carboxy-5-aminoimidazole ribonucleotide 50 mM Tris-HCl (pH 8.0), at 30°C Treponema denticola
77
-
 CO2 50 mM Tris-HCl (pH 8.0), at 30°C Treponema denticola

pI Value

Organism Comment pI Value Maximum pI Value
Treponema denticola calculated from amino acid sequence
-
 7.7

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.4
-
 5-aminoimidazole ribonucleotide 50 mM Tris-HCl (pH 8.0), at 10°C Treponema denticola
33
-
 4-carboxy-5-aminoimidazole ribonucleotide 50 mM Tris-HCl (pH 8.0), at 30°C Treponema denticola