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Literature summary for 4.1.1.20 extracted from
- Analysis of catalytic determinants of diaminopimelate and ornithine decarboxylases using alternate substrates (2011), Biochim. Biophys. Acta, 1814, 1113-1119.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-Lysine | a small amount of decarboxlation occurs | Mycobacterium tuberculosis |  |
| L,L-2,6-diaminoheptanedioate | no detectable amounts of decarboxylation products | Mycobacterium tuberculosis | |
| L-lysine | no detectable amounts of decarboxylation products | Mycobacterium tuberculosis | |
| LL-2,6-Diaminoheptanedioate | - |
Mycobacterium tuberculosis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
meso-2,6-diaminoheptanedioate | pH 7.8, 25°C | Mycobacterium tuberculosis | |
| 0.43 | - |
DL-2,6-Diaminoheptanedioate | in 100 mM TEA-HCl pH 7.8, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 1 | 7 | D,D-2,6-diaminoheptanedioate | pH 7.8, 25°C | Mycobacterium tuberculosis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
| Mycobacterium tuberculosis | P9WIU7 | - |
- |
| Mycobacterium tuberculosis H37Rv | P9WIU7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D,D-2,6-diaminoheptanedioate | - |
Mycobacterium tuberculosis | L-lysine + CO2 | - |
? | |
| D,D-2,6-diaminoheptanedioate | - |
Mycobacterium tuberculosis H37Rv | L-lysine + CO2 | - |
? | |
| DD-2,6-diaminoheptanedioate | DD-2,6-diaminoheptanedioate is a reasonable substrate for DAPDC but with an about 100fold lower kcat/Km value than DL-DAP | Mycobacterium tuberculosis | L-lysine + CO2 | - |
? | |
| DL-2,6-diaminoheptanedioate | - |
Mycobacterium tuberculosis | L-lysine + CO2 | - |
? | |
| meso-2,6-Diaminoheptanedioate | - |
Mycobacterium tuberculosis | L-Lysine + CO2 | - |
? | |
| meso-2,6-Diaminoheptanedioate | commercial meso-2,6-diaminoheptanedioate is a mixture of 50% DL-2,6-diaminoheptanedioate, 25% DD-2,6-diaminoheptanedioate and 25% LL-2,6-diaminoheptanedioate | Mycobacterium tuberculosis | L-Lysine + CO2 | - |
? | |
| meso-2,6-Diaminoheptanedioate | - |
Mycobacterium tuberculosis H37Rv | L-Lysine + CO2 | - |
? | |
| additional information | the reaction catalyzed by diaminopimelate decarboxylase on D stereocenters could be a concerted, backside electrophilic substitution reaction with simultaneous CO2 loss from Calpha and proton donation to the backside of Calpha from Lys72 | Mycobacterium tuberculosis | ? | - |
? | |
| additional information | the reaction catalyzed by diaminopimelate decarboxylase on D stereocenters could be a concerted, backside electrophilic substitution reaction with simultaneous CO2 loss from Calpha and proton donation to the backside of Calpha from Lys72 | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DAPDC | - |
Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.8 | - |
D,D-2,6-diaminoheptanedioate | pH 7.8, 25°C | Mycobacterium tuberculosis | |
| 1.8 | - |
DD-2,6-diaminoheptanedioate | in 100 mM TEA-HCl pH 7.8, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 7.1 | - |
meso-2,6-diaminoheptanedioate | pH 7.8, 25°C | Mycobacterium tuberculosis | |
| 7.1 | - |
meso-2,6-diaminoheptanedioate | in 100 mM TEA-HCl pH 7.8, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 7.1 | - |
DL-2,6-Diaminoheptanedioate | in 100 mM TEA-HCl pH 7.8, temperature not specified in the publication | Mycobacterium tuberculosis | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Mycobacterium tuberculosis | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.6 | - |
L,L-2,6-diaminoheptanedioate | pH 7.8, 25°C | Mycobacterium tuberculosis | |
| 1.6 | - |
LL-2,6-Diaminoheptanedioate | in 100 mM TEA-HCl pH 7.8, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 1.8 | - |
L-lysine | pH 7.8, 25°C | Mycobacterium tuberculosis | |
| 1.8 | - |
L-lysine | in 100 mM TEA-HCl pH 7.8, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 26 | - |
D-Lysine | pH 7.8, 25°C | Mycobacterium tuberculosis | |
| 26 | - |
D-Lysine | in 100 mM TEA-HCl pH 7.8, temperature not specified in the publication | Mycobacterium tuberculosis | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
D,D-2,6-diaminoheptanedioate | pH 7.8, 25°C | Mycobacterium tuberculosis | |
| 16 | - |
DL-2,6-Diaminoheptanedioate | in 100 mM TEA-HCl pH 7.8, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 18 | - |
meso-2,6-diaminoheptanedioate | pH 7.8, 25°C | Mycobacterium tuberculosis | |
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