Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain DE3 | Selenomonas ruminantium |
General Stability | Organism |
---|---|
at 50°C in absence of PALP lost of 90% enzyme activity within 10 min, no enzyme activity after heating at 70°C for 60 min in presence of PALP | Selenomonas ruminantium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DL-alpha-difluoromethylarginine | - |
Selenomonas ruminantium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.6 | - |
L-arginine | - |
Selenomonas ruminantium | |
50 | - |
L-lysine | - |
Selenomonas ruminantium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
SDS-PAGE | Selenomonas ruminantium |
120000 | - |
gel filtration | Selenomonas ruminantium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Selenomonas ruminantium | Q5NT93 | - |
- |
Purification (Comment) | Organism |
---|---|
2024fold after final purification step with MonoQ HR 5/5 | Selenomonas ruminantium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00738 | - |
crude extract | Selenomonas ruminantium |
0.01104 | - |
heat treatment | Selenomonas ruminantium |
0.02394 | - |
after purification with DEAE-Toyopearl 650M | Selenomonas ruminantium |
0.0381 | - |
after purification with DEAE-5PW | Selenomonas ruminantium |
1.026 | - |
at the semi-final step of purification with HA-1000 column chromatography | Selenomonas ruminantium |
14.94 | - |
final step of purification with MonoQ HR 5/5 | Selenomonas ruminantium |
Storage Stability | Organism |
---|---|
4°C, 1 month, no lost of enzyme activity, pH 6.0-7.5 | Selenomonas ruminantium |
50°C, absence of PALP, 10 min, only 10% residual activity | Selenomonas ruminantium |
70°C, presence of PALP, 60 min, no residual activity | Selenomonas ruminantium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine | assay at pH 6.5, 10 min, 50°C | Selenomonas ruminantium | agmatine + CO2 | - |
? | |
L-lysine | assay at pH 6.5, 10 min, 50°C | Selenomonas ruminantium | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Selenomonas ruminantium |
Synonyms | Comment | Organism |
---|---|---|
ADC | - |
Selenomonas ruminantium |
arginine decarboxylase | - |
Selenomonas ruminantium |
L-Arginine decarboxylase | - |
Selenomonas ruminantium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Selenomonas ruminantium |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | after 10 min incubation at 50°C 4fold higher enzyme activity than at 40°C, after 10 min incubation at 55°C 7fold higher enzyme activity than at 40°C, after 10 min incubation at 60°C 10fold higher enzyme activity than at 40°C, after 10 min incubation at 70°C 1.2fold higher enzyme activity than at 40°C | Selenomonas ruminantium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
without pyridoxal 5'-phosphate, 50% redsidual enzyme activity | Selenomonas ruminantium |
60 | - |
with pyridoxal 5'-phosphate, 54% residual enzyme activity | Selenomonas ruminantium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at pH 6.5, 10 min, 50°C | Selenomonas ruminantium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | 80% enzyme activity at pH 7.0, 70% enzyme activity at pH 8.0, 50% enzyme activity at pH 9.0 | Selenomonas ruminantium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | more than 80% enzyme activity, decreased enzyme activity at higher pH | Selenomonas ruminantium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Selenomonas ruminantium |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
DL-alpha-difluoromethylarginine | - |
Selenomonas ruminantium |