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Literature summary for 4.1.1.19 extracted from

  • Liao, S.; Poonpairoj, P.; Ko, K.; Takatuska, Y.; Yamaguchi, Y.; Abe, N.; Kaneko, J.; Kamio, Y.
    Occurrence of agmatine pathway for putrescine synthesis in Selenomonas ruminatium (2008), Biosci. Biotechnol. Biochem., 72, 445-455.
    View publication on PubMed
Search for more literature for 4.1.1.19

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain DE3 Selenomonas ruminantium

General Stability

General Stability Organism
at 50°C in absence of PALP lost of 90% enzyme activity within 10 min, no enzyme activity after heating at 70°C for 60 min in presence of PALP Selenomonas ruminantium

Inhibitors

Inhibitors Comment Organism Structure
DL-alpha-difluoromethylarginine
-
 Selenomonas ruminantium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.6
-
 L-arginine
-
 Selenomonas ruminantium
50
-
 L-lysine
-
 Selenomonas ruminantium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 SDS-PAGE Selenomonas ruminantium
120000
-
 gel filtration Selenomonas ruminantium

Organism

Organism UniProt Comment Textmining
Selenomonas ruminantium Q5NT93
-
-

Purification (Commentary)

Purification (Comment) Organism
2024fold after final purification step with MonoQ HR 5/5 Selenomonas ruminantium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00738
-
 crude extract Selenomonas ruminantium
0.01104
-
 heat treatment Selenomonas ruminantium
0.02394
-
 after purification with DEAE-Toyopearl 650M Selenomonas ruminantium
0.0381
-
 after purification with DEAE-5PW Selenomonas ruminantium
1.026
-
 at the semi-final step of purification with HA-1000 column chromatography Selenomonas ruminantium
14.94
-
 final step of purification with MonoQ HR 5/5 Selenomonas ruminantium

Storage Stability

Storage Stability Organism
4°C, 1 month, no lost of enzyme activity, pH 6.0-7.5 Selenomonas ruminantium
50°C, absence of PALP, 10 min, only 10% residual activity Selenomonas ruminantium
70°C, presence of PALP, 60 min, no residual activity Selenomonas ruminantium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine assay at pH 6.5, 10 min, 50°C Selenomonas ruminantium agmatine + CO2
-
 ?
L-lysine assay at pH 6.5, 10 min, 50°C Selenomonas ruminantium ?
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Selenomonas ruminantium

Synonyms

Synonyms Comment Organism
ADC
-
 Selenomonas ruminantium
arginine decarboxylase
-
 Selenomonas ruminantium
L-Arginine decarboxylase
-
 Selenomonas ruminantium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Selenomonas ruminantium

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70 after 10 min incubation at 50°C 4fold higher enzyme activity than at 40°C, after 10 min incubation at 55°C 7fold higher enzyme activity than at 40°C, after 10 min incubation at 60°C 10fold higher enzyme activity than at 40°C, after 10 min incubation at 70°C 1.2fold higher enzyme activity than at 40°C Selenomonas ruminantium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 without pyridoxal 5'-phosphate, 50% redsidual enzyme activity Selenomonas ruminantium
60
-
 with pyridoxal 5'-phosphate, 54% residual enzyme activity Selenomonas ruminantium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at pH 6.5, 10 min, 50°C Selenomonas ruminantium

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9 80% enzyme activity at pH 7.0, 70% enzyme activity at pH 8.0, 50% enzyme activity at pH 9.0 Selenomonas ruminantium

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 7.5 more than 80% enzyme activity, decreased enzyme activity at higher pH Selenomonas ruminantium

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Selenomonas ruminantium

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.8
-
 DL-alpha-difluoromethylarginine
-
 Selenomonas ruminantium