Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 as GST-fusion protein | Dianthus caryophyllus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 0.1 mM, 70% loss of activity | Dianthus caryophyllus | |
Cu2+ | 0.1 mM, 70% loss of activity | Dianthus caryophyllus | |
additional information | activity not affected by Mg2+ or Ca2+ at 0.1 mM | Dianthus caryophyllus | |
spermine | 1 mM, 30% loss of activity | Dianthus caryophyllus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.077 | - |
L-Arg | - |
Dianthus caryophyllus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
4 * 78000, subunit mass calculated from the deduced amino acid sequence, SDS-PAGE | Dianthus caryophyllus |
327000 | - |
gel filtration | Dianthus caryophyllus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine | Dianthus caryophyllus | key enzyme in the biosynthesis of polyamines, primarily responsible for the biosynthesis of putrescine in non-dividing elongation cells, plays a role in stress response | agmatine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dianthus caryophyllus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein using GST-tag | Dianthus caryophyllus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine | - |
Dianthus caryophyllus | agmatine + CO2 | - |
? | |
L-arginine | key enzyme in the biosynthesis of polyamines, primarily responsible for the biosynthesis of putrescine in non-dividing elongation cells, plays a role in stress response | Dianthus caryophyllus | agmatine + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 78000, subunit mass calculated from the deduced amino acid sequence, SDS-PAGE | Dianthus caryophyllus |