Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas dacunhae |
Crystallization (Comment) | Organism |
---|---|
microbatch-under-oil method, using 100 mM MgCl2, 100 mM Tris, pH 8.5, 25% (v/v) polyethylene glycol (PEG) 400, and 10% (v/v) glycerol | Pseudomonas dacunhae |
Protein Variants | Comment | Organism |
---|---|---|
R37A | the mutant shows reduced activity | Pseudomonas dacunhae |
S298R | the mutant shows very low activity | Pseudomonas dacunhae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.4 | - |
L-Asp | wild type enzyme, in 50 mM potassium phosphate, at pH 7.0 and 37°C | Pseudomonas dacunhae | |
16.9 | - |
L-Asp | mutant enzyme R37A, in 50 mM potassium phosphate, at pH 7.0 and 37°C | Pseudomonas dacunhae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas dacunhae | Q845W8 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and Sepharose CL-6B resin gel filtration | Pseudomonas dacunhae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Asp | - |
Pseudomonas dacunhae | L-Ala + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homododecamer | x-ray crystallography | Pseudomonas dacunhae |
Synonyms | Comment | Organism |
---|---|---|
ABDC | - |
Pseudomonas dacunhae |
aspartate 4-carboxylyase | - |
Pseudomonas dacunhae |
aspartate-beta-decarboxylase | - |
Pseudomonas dacunhae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
L-Asp | mutant enzyme R37A, in 50 mM potassium phosphate, at pH 7.0 and 37°C | Pseudomonas dacunhae | |
4.3 | - |
L-Asp | wild type enzyme, in 50 mM potassium phosphate, at pH 7.0 and 37°C | Pseudomonas dacunhae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Pseudomonas dacunhae |