Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 1 mM, 7% activation | Pseudomonas sp. | |
DTT | 1 mM, 7% activation | Pseudomonas sp. | |
EDTA | 10-20 mM, 10% activation | Pseudomonas sp. |
Application | Comment | Organism |
---|---|---|
industry | Asd is a major enzyme used in the industrial production of L-alanine | Pseudomonas sp. |
synthesis | Asd is a major enzyme used in the industrial production of L-alanine | Pseudomonas sp. |
Cloned (Comment) | Organism |
---|---|
gene asd, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of the C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, 14% inhibition | Pseudomonas sp. | |
Co2+ | 10 mM, over 90% inhibition | Pseudomonas sp. | |
Cu2+ | 10 mM, over 90% inhibition | Pseudomonas sp. | |
Fe2+ | 10 mM, over 90% inhibition | Pseudomonas sp. | |
Hg2+ | 10 mM, complete inhibition | Pseudomonas sp. | |
Mg2+ | 10 mM, 64% inhibition | Pseudomonas sp. | |
Mn2+ | 10 mM, over 90% inhibition | Pseudomonas sp. | |
Ni2+ | 10 mM, over 90% inhibition | Pseudomonas sp. | |
Zn2+ | 10 mM, over 90% inhibition | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.5 | - |
L-aspartate | pH 6.8, 37°C, recombinant enzyme | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no divalent metal ion required for catalysis | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59243 | - |
12 * 63000, pH 7.0, SDS-PAGE, 12 * 59243, sequence calculation | Pseudomonas sp. |
59243 | - |
2 * 63000, pH 7.0, SDS-PAGE, 2 * 59243, sequence calculation | Pseudomonas sp. |
63000 | - |
12 * 63000, pH 7.0, SDS-PAGE, 12 * 59243, sequence calculation | Pseudomonas sp. |
63000 | - |
2 * 63000, pH 7.0, SDS-PAGE, 2 * 59243, sequence calculation | Pseudomonas sp. |
760000 | - |
dodedamer, pH 5.0, recombinant enzyme, gel filtration | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate | Pseudomonas sp. | - |
L-alanine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | Q53IZ1 | strain ATCC 19121 | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
280 | - |
purified recombinant enzyme, pH 5.0, 37°C | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate | - |
Pseudomonas sp. | L-alanine + CO2 | - |
? | |
L-aspartate | the decarboxylation activity is 2477fold higher than the aminotransferase activity of the enzyme, highly preferred substrate | Pseudomonas sp. | L-alanine + CO2 | - |
? | |
additional information | D-aspartate, L-lysine, L-asparagine, L-glutamate, and L-glutamine are poor substrates in beta-elimination and transamination reaction catalyzes by the enzyme, substrate specificity, overview | Pseudomonas sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 63000, pH 7.0, SDS-PAGE, 2 * 59243, sequence calculation | Pseudomonas sp. |
dodecamer | 12 * 63000, pH 7.0, SDS-PAGE, 12 * 59243, sequence calculation | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
Asd | - |
Pseudomonas sp. |
L-aspartate 4-decarboxylase | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Pseudomonas sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 45 | 92% of maximal activity at 40°C, maximal activity at 45°C | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 8.5 | purified recombinant enzyme, 4°C, 1 day, completely stable | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Pseudomonas sp. |