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Literature summary for 4.1.1.12 extracted from

  • Wang, N.C.; Lee, C.Y.
    Enhanced transaminase activity of a bifunctional L-aspartate 4-decarboxylase (2007), Biochem. Biophys. Res. Commun., 356, 368-373.
    View publication on PubMed
Search for more literature for 4.1.1.12

Cloned(Commentary)

Cloned (Comment) Organism
gene asd, DNA and amino acid sequence determination and analysis, sequence comparisons Pseudomonas sp.

Protein Variants

Protein Variants Comment Organism
D360P site-directed mutagenesis, the mutant shows similar 4-decarboxylation activity but reduced aminotransferase compared to the wild-type enzyme Pseudomonas sp.
F204W site-directed mutagenesis, the mutant shows slightly reduced 4-decarboxylation activity but highly increased aminotransferase compared to the wild-type enzyme Pseudomonas sp.
H123Y site-directed mutagenesis, the mutant shows reduced 4-decarboxylation activity and slightly increased aminotransferase compared to the wild-type enzyme Pseudomonas sp.
H337R site-directed mutagenesis, the mutant shows reduced 4-decarboxylation activity but similar aminotransferase compared to the wild-type enzyme Pseudomonas sp.
K347R site-directed mutagenesis, the mutant shows similar 4-decarboxylation activity but reduced aminotransferase compared to the wild-type enzyme Pseudomonas sp.
M178L site-directed mutagenesis, the mutant shows reduced 4-decarboxylation activity but unaltered aminotransferase compared to the wild-type enzyme Pseudomonas sp.
V475L site-directed mutagenesis, the mutant shows slightly reduced 4-decarboxylation activity but unaltered aminotransferase compared to the wild-type enzyme Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate Pseudomonas sp.
-
 L-alanine + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. Q53IZ1 strain ATCC 19121
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 4-decarboxylation activity and aminotransferase activity of mutant enzymes, overview Pseudomonas sp.
0.06
-
 recombinant wild-type enzyme, aminotransferase activity Pseudomonas sp.
128.8
-
 recombinant wild-type enzyme, 4-decarboxylation activity Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate
-
 Pseudomonas sp. L-alanine + CO2
-
 ?
additional information Asd is a bifunctional enzyme performing decarboxylation and aminotransferase activities on L-aspartate Pseudomonas sp. ?
-
 ?

Synonyms

Synonyms Comment Organism
Asd
-
 Pseudomonas sp.
L-aspartate 4-decarboxylase
-
 Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 assay at Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Pseudomonas sp.